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2 protocols using sodium nitrate

1

Antioxidant and Hepatoprotective Assays

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Adenine, aluminum chloride, potassium hydroxide, sodium nitrate, and Folin–Ciocalteu reagent were purchased from Carl-Roth (Germany). 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), (2′, 2′-azobis (2-methylpropionamidine) dihydrochloride) (AAPH), gallic acid, quercetin, ascorbic acid, Tris, hydrogen peroxide, trichloroacetic acid, ferric chloride, and hydrogen chloride were purchased from Sigma–Aldrich (Germany). Captopril was purchased from Denk Pharma GmbH and Co., KG (Germany). Thiobarbituric acid was from Fluka. Sodium chloride and potassium chloride were purchased from BDH Chemicals Ltd. (England). Creatinine, urea, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) kits were purchased at SGM Italia (Roma). Sodium thiopental was from Samarth Life Sciences Pvt Ltd. (Mumbai).
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2

Fungal Bioconversion of Agricultural Waste

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MB (98%),
CR (82%), calcium sulfate dihydrate
(98%), and streptomycin sulfate salt were obtained from Sigma-Aldrich
(Germany, USA). Hydrochloric acid (37%), acetic acid (100%), sodium
chloride (≥95%), malt extract agar, sodium nitrate (>99%),
and glucose monohydrate (microbiology grade) were purchased from Carl
Roth GmbH + Co. KG (Germany). Sodium hydroxide (p.a.) was obtained
from Merck. Ethanol (≥96%), formic acid (≥99%, HiPerSolv
CHROMANORM), and acetonitrile (≥99.9%, HiPerSolv CHROMANORM)
were provided by VWR (Germany). Ampicillin sodium salt was acquired
from AppliChem GmbH (Germany). Hemp shives were purchased from Hemparade
(The Netherlands). Brown millet was provided by Mühle Schlingemann
(Germany). Yeast extract and Gibco casamino acids were obtained from
Ohly GmbH (Germany) and Life Technologies (USA), respectively. DIW
was used for stock solution and fungi preparation.
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