Western blot analysis was performed with p21, p53, p-ser15 p53, p-H2AX, PARP (Santa Cruz), HdmX (Bethyl) and β-actin antibodies (Sigma). Cells were treated as indicated and washed in PBS. Cells were centrifuged and lysed with a Triton containing lysis buffer. Protein lysates (50 μg per lane) were electrophoresed on SDS-polyacrylamide gels and then transferred to PVDF membranes (Millipore) using a wet transfer apparatus (Bio-Rad). The membranes were blocked, incubated with the indicated primary antibodies at 4°C overnight, and then the appropriate HRP conjugated secondary antibody. Protein bands were visualized by autoradiography after incubation with enhanced chemiluminescence reagent (Millipore).
P ser15 p53
Manufactured by Santa Cruz Biotechnology
P-ser15 p53 is an antibody that recognizes the phosphorylated form of the p53 protein at serine 15. The p53 protein is a crucial regulator of cellular responses to various stresses, including DNA damage. Phosphorylation of p53 at serine 15 is an important post-translational modification that can affect the activity and stability of the p53 protein.
Automatically generated - may contain errors
Lab products found in correlation
Wet transfer apparatus, by Bio-Rad (1 mentions)
Enhanced chemiluminescence reagent, by Merck Group (1 mentions)
P h2ax, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
Neocarzinostatin ncs, by Merck Group (1 mentions)
Carboxyfluorescein diacetate succinimidyl ester cfse, by Merck Group (1 mentions)
Fitc conjugated anti brdu antibody, by BD (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
Nutlin 3, by MedChemExpress (1 mentions)
Gsk2830371, by MedChemExpress (1 mentions)
P38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions)
γh2ax, by Merck Group (1 mentions)
Alexa fluor labelled secondary antibody, by Thermo Fisher Scientific (1 mentions)
P38 mapk, by Cell Signaling Technology (1 mentions)
Doxorubicin hydrochloride, by Merck Group (1 mentions)
2 protocols using p ser15 p53
1
Western Blot Analysis of Cell Signaling
2
Evaluating DNA Damage and Cell Cycle Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used: WIP1 (sc-130655), p53 (sc-6243), TFIIH (sc-293), importin (sc-137016), p21 (sc-397) from Santa Cruz; pSer15-p53 (#9284), γH2AX (#9718), p38 MAPK Thr180/Tyr182 (#9216S) and p38 MAPK (#9212) from Cell Signaling Technology); γH2AX (05-636, Millipore); MDM2 (Calbiochem); Alexa Fluor-labelled secondary antibodies (Life Technologies); anti-BrdU FITC-conjugated antibody (#347583, BD Biosciences) and anti-pSer10-H3 antibody (Upstate). Doxorubicin hydrochloride (Sigma), GSK2830371 and nutlin-3 (both MedChem Express) were diluted in DMSO and used at indicated doses. Resazurin, neocarzinostatin (NCS) and carboxyfluorescein diacetate succinimidyl ester (CFSE) were purchased from Sigma.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!