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2 protocols using trypticase peptone

1

Microbial Analysis of Anaerobic Gut Communities

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Trypticase peptone, peptone, yeast extract, meat extract, Tween 80, and Gram staining kits were supplied by Sigma‐Aldrich (St. Louis, MO, USA). Monosugar standards including glucose and xylose, a succinic acid standard, and short‐chain fatty acid standards including acetic acid, formic acid, lactic acid, propionic acid, butyric acid, and valeric acid were also supplied by Sigma‐Aldrich (St. Louis, MO, USA). Defibrinated sheep blood and Schaedler anaerobe agar were purchased from Oxoid (Basingstoke, UK). Anaerocult A was supplied by Merck (Darmstadt, Germany). Quick‐DNA Fungal/Bacterial Microprep kit was supplied by Zymo Research (Irvine, CA, USA) and QIAquick gel extraction kit from QIAGEN (Hilden, Germany). Cysteine hydrochloride and reagents for molecular biology including DreamTaq PCR Master Mix (2X) and nuclease‐free water were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All chemicals were of analytical grade or higher quality.
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2

Topical Antimicrobial Gel Formulation

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Diclofenac sodium, betamethasone dipropionate, chlorhexidine digluconate (20% solution), polyethylene glycol (PEG) 400, trifluoroacetic acid (≥99.0%), Hanks buffer, protease peptone, trypticase peptone, yeast extract, KCl, haemin, vitamin K1, (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) (MTT), L-cysteine, HCl, glucose, Mueller Hinton agar and Mueller Hinton broth were all obtained from Sigma-Aldrich, Gillingham, UK. Phosphate buffered saline (PBS) tablets, Dulbecco's modified Eagle's medium, fetal calf serum (FCS), L-glutamine, amphotericin B, penicillin G, streptomycin sulfate, HPLC-grade water, acetonitrile and ethanol were obtained from Fisher Scientific (Loughborough, UK). Carbopol 917 (CP) was a gift from Noveon Inc., Cleveland, U.S.A. Hydroxypropylmethyl cellulose was a gift from Shin-Etsu Chemical Co. (Tokyo, Japan). Primary human gingival fibroblasts were purchased from LGC standards, UK. Pig heads were obtained from a local abattoir and used within 2 h of slaughter.
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