Steady-state fluorescence experiments were carried out using a Photon Technology International (PTI, Germany) LPS-220B spectrofluorometer, equipped with a monochromator in the range of 200–700 nm. Time-resolved fluorescence measurements were performed with a Time Master fluorescence lifetime spectrometer TM 2/2003 from PTI, using a hydrogen/nitrogen flash lamp as the excitation source. The kinetic traces were fitted by monoexponential decay functions, using a deconvolution procedure to separate them from the lamp pulse profile. Emission measurements were performed in the region of 330–600 nm. The absorbance of the solutions was adjusted at ca. 0.08 at 308 nm. All measurements were performed at room temperature using 10 mm × 10 mm quartz cells of 4 mL capacity, under aerobic conditions.
Lps 220b spectrofluorometer
Manufactured by Horiba
Sourced in Germany
The LPS-220B is a spectrofluorometer manufactured by Horiba. It is a laboratory instrument used for the analysis of fluorescent samples. The LPS-220B measures the emission spectrum of a sample when excited by a light source.
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Lab products found in correlation
2 protocols using lps 220b spectrofluorometer
1
Steady-State and Time-Resolved Fluorescence Measurements
2
Characterization of Photophysical Properties
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A Shimadzu UV-2101PC spectrophotometer was employed to obtain the UV/Vis absorption spectra.
Steady-state and time-resolved fluorescence experiments were performed on a Photon Technology International (PTI) LPS-220B spectrofluorometer and on an EasyLife V spectrofluorometer from OBB, respectively. In the case of time-resolved fluorescence, the excitation source was equipped with a pulsed LED (λexc=460 nm); the residual excitation signal was filtered in emission using a cut-off filter (50% transmission at 475 nm). Monoexponential decay functions that use a deconvolution procedure to separate from the lamp pulse profile provided the fitted kinetic traces.
A pulsed Nd: YAG SL404G-10 Spectron Laser Systems at the excitation wavelengths of 355 and 532 nm for RFTA and EY, respectively, was employed to carry out the laser flash photolysis (LFP) experiments. The energy of the single pulses (∼10 ns duration) was lower than 15 mJ pulse -1 . The laser flash photolysis system was formed by the above-mentioned pulsed laser, a pulsed Lo255 Oriel Xenon lamp, a 77,200 Oriel monochromator, an Oriel photomultiplier tube (PMT) housing, a 70,705 PMT power supply and a TDS-640A Tektronix oscilloscope.
Steady-state and time-resolved fluorescence experiments were performed on a Photon Technology International (PTI) LPS-220B spectrofluorometer and on an EasyLife V spectrofluorometer from OBB, respectively. In the case of time-resolved fluorescence, the excitation source was equipped with a pulsed LED (λexc=460 nm); the residual excitation signal was filtered in emission using a cut-off filter (50% transmission at 475 nm). Monoexponential decay functions that use a deconvolution procedure to separate from the lamp pulse profile provided the fitted kinetic traces.
A pulsed Nd: YAG SL404G-10 Spectron Laser Systems at the excitation wavelengths of 355 and 532 nm for RFTA and EY, respectively, was employed to carry out the laser flash photolysis (LFP) experiments. The energy of the single pulses (∼10 ns duration) was lower than 15 mJ pulse -1 . The laser flash photolysis system was formed by the above-mentioned pulsed laser, a pulsed Lo255 Oriel Xenon lamp, a 77,200 Oriel monochromator, an Oriel photomultiplier tube (PMT) housing, a 70,705 PMT power supply and a TDS-640A Tektronix oscilloscope.
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