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Streptavidin immunocaps

Manufactured by Thermo Fisher Scientific
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Streptavidin ImmunoCAPs are solid-phase immunoassay platforms designed for the detection and quantification of various analytes. They utilize the high-affinity interaction between streptavidin and biotin to capture and immobilize target molecules on a solid surface, enabling efficient and reliable analysis.

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Lab products found in correlation

Phadia 250, by Thermo Fisher Scientific (1 mentions) Phadia 100, by Thermo Fisher Scientific (1 mentions) Recombinant sars cov 2 nucleocapsid protein, by RayBiotech (1 mentions)

2 protocols using streptavidin immunocaps

1

Optimizing Allergen-Loaded Immunocaps

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Streptavidin ImmunoCAPs (Thermo Fisher Scientific, Portage, MI) were loaded and incubated on a Phadia 100, with the biotinylated allergen at amounts ranging from 0.5 to 10 µg/CAP. Two different human plasma samples from individuals allergic to the allergen (that had been originally tested for IgE binding to 2–3 µg/CAP) were selected for optimization experiments. Their IgE levels to the allergen-loaded CAPs were measured in a Phadia 250 following manufacturer’s instructions (Thermo Fisher Scientific, Portage, MI) to select optimal amount of biotinylated allergen to be loaded to the Streptavidin ImmunoCAPs.
Pomés A., Schulten V., Glesner J., da Silva Antunes R., Sutherland A., Bacharier L.B., Beigelman A., Busse P., Frazier A, & Sette A. (2021). IgE and T Cell Reactivity to a Comprehensive Panel of Cockroach Allergens in Relation to Disease. Frontiers in Immunology, 11, 621700.
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2

SARS-CoV-2 Antibody Detection Assay

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Recombinant SARS-CoV-2 S1 receptor-binding domain subunit (RBD) and recombinant SARS-CoV-2 nucleocapsid protein which had been expressed in HEK293 cells were purchased from RayBiotech (Peachtree Corners, GA). Human coronavirus (HCoV-229E) spike protein (S1 subunit, His Tag) and human coronavirus (HCoV-OC43) spike protein (S1 subunit, His Tag), protein were purchased from Sino Biological (Wayne, PA). The recombinant monoclonal anti-SARS-CoV-2 spike glycoprotein S1 antibody (CR3022) was purchased from Abcam (Cambridge, MA). Streptavidin ImmunoCAPs and tetanus toxoid ImmunoCAPs, as well as ImmunoCAPs to measure total IgG, were purchased from Thermo Fisher Scientific (Portage, MI). Galactose-α-1,3-galactose-β-1,4-GlcNAc-HSA (14 atom spacer) was purchased from Dextra (Reading, UK).
Keshavarz B., Wiencek J.R., Workman L.J., Straesser M.D., Muehling L.M., Canderan G., Drago F., Bonham C.A., Sturek J.M., Ramani C., McNamara C.A., Woodfolk J.A., Kadl A., Platts-Mills T.A, & Wilson J.M. (2021). Quantitative Measurement of IgG to Severe Acute Respiratory Syndrome Coronavirus-2 Proteins Using ImmunoCAP. International Archives of Allergy and Immunology, 182(5), 417-424.
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