Following antibodies were used: anti-GAPDH (HRP-60004, Proteintech), anti-6∗His (ab18184, Abcam), anti-GFP (11814460001, Roche), anti-Myc (2278S, Cell Signaling Technology), anti-ACBD3 (H00064746-B01P, Abnova), anti-P-PKA-substrate(9621S, Cell Signaling Technology), anti-Golgin97 (13192, Cell Signaling Technology), anti-mCherry (ab167453, Abcam), anti-PKA-Cα (#4782, Cell Signaling Technology), anti-PKA-RIIα (612242, BD), anti-γ-COP (sc-393615, Santa Cruz), anti-GM130 (610822, BD Bioscience), anti-SNAP tag (P9310S, NEB), anti-Clathrin HC (ab21679, Abcam), anti-ARF1 (NB300-505, Novus), anti-β-Actin (3700S, Cell Signaling), and anti-α-Tubulin (3873S, Cell Signaling). Anti-Rabbit Alexa Fluor 488 (A21441), Alexa Fluor 568 (A10042), Alexa Fluor 647 (A21245), and anti-Mouse Alexa Fluor 488 (A21200), Alexa Fluor 568 (A10037), and Alexa Fluor 647 (A21236) for immunofluorescence staining were obtained from ThermoFisher. D/D Solubilizer (635054) was purchased from Clontech. Forskolin (F6886) was purchased from Sigma.
Anti snap tag
Manufactured by New England Biolabs
The Anti-SNAP tag is a tool used in molecular biology research. It serves as a detection reagent for the SNAP-tag, a protein tag that can be fused to other proteins of interest and then labeled with a fluorescent dye. The Anti-SNAP tag is designed to specifically bind to the SNAP-tag, allowing for the visualization and detection of the tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Ab21679, by Abcam (1 mentions)
Ab167453, by Abcam (1 mentions)
Ab18184, by Abcam (1 mentions)
Anti mcherry, by Abcam (1 mentions)
Anti golgin97, by Cell Signaling Technology (1 mentions)
Alexa fluor 568 a10037, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 a21236, by Thermo Fisher Scientific (1 mentions)
Anti myc, by Cell Signaling Technology (1 mentions)
Anti pka c α, by Cell Signaling Technology (1 mentions)
Forskolin f6886, by Merck Group (1 mentions)
Anti α tubulin, by Cell Signaling Technology (1 mentions)
D d solubilizer, by Takara Bio (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti gm130, by BD (1 mentions)
Anti gαo, by Santa Cruz Biotechnology (1 mentions)
Anti gαs, by Santa Cruz Biotechnology (1 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti halotag, by Promega (1 mentions)
2 protocols using anti snap tag
1
Immunofluorescence Staining of Cellular Proteins
2
GPCR-GIRK4 Concatemers in HEK-293T Cells
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Untransfected HEK-293T cells or HEK-293T cells transiently transfected with GPCR-GIRK4 concatemers were centrifuged and mixed with an equal volume of loading buffer containing 4% SDS and 10% β-mercaptoethanol. Samples were then run using standard SDS-PAGE procedures on Invitrogen NuPAGE 4–12% Bis-Tris gels and transferred onto PVDF membranes. Western Blot was performed using an anti-SNAP-tag (NEB), anti-HaloTag (Promega), anti-Gαi1 (abcam, ab140125), anti-Gαi2 (abcam, ab157204), anti-Gαo (Santa Cruz Biotechnology, sc-13532), or anti-Gαs (Santa Cruz Biotechnology, sc-383).
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