Most of the phenotypes reported here can be counted under a dissecting microscope. When closer examination was necessary, we used a Leica DM5000B compound microscope fitted with a Retiga 2000R camera. To stitch together individual images, we used the MosaicJ plugin [106 (link)] with ImageJ software. To view adult worms under the compound scope, we mounted them on agarose pads by picking individuals into a drop of 100 mM sodium azide.
2000r camera
Manufactured by Leica
The 2000R camera is a compact and versatile laboratory equipment designed for various scientific and research applications. It features a high-resolution sensor, advanced optics, and robust construction, making it a reliable tool for accurate image capture and data collection. The 2000R's core function is to provide precise and detailed visual documentation of specimens, materials, or experimental setups within a controlled laboratory environment.
Automatically generated - may contain errors
2 protocols using 2000r camera
1
Microscopic Worm Phenotype Analysis
2
Counting Cell Nuclei in Hermaphrodites
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Hermaphrodites were aged in small populations of 30 per plate on either ascr#10 or control plates. At the indicated times, they were stained with DAPI as described (21 (link)) using a variation of the protocol by Pepper et al. (69 (link)). Briefly, following washes in M9 and fixation with 95% ethanol, animals were incubated with Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA) and mounted on 2% agarose pads for visualization. We counted the number of nuclei in the progenitor zone, as defined by Crittenden et al. (70 (link)). The slides were imaged on a Leica DM5000B microscope using a Retiga 2000R camera.
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