Ferric cyanide

The study involves use of analytical grade solvents i.e. n-hexane; chloroform; acetone; ethyl acetate; ethanol; methanol and dimethyl sulfoxide (DMSO) purchased from Sigma (Sigma-Aldrich, USA). The reagents include gallic acid; quercetin; 2,2-diphenyl-1-picryl-hydrazyl (DPPH); potassium acetate; aluminum chloride; Folin-Ciocalteu (F-C) reagent; sodium carbonate; ascorbic acid; ammonium molybdate; sodium phosphate; sulfuric acid; ferric cyanide; trichloroacetic acid and potassium ferricyanide procured from Merck (Merck KGaA, Germany), rutin; myricetin; caffiec acid;apigenin;kaempferol; catechin; and caffeic acid were purchased from Sigma (Sigma-Aldrich, Germany). Sabouraud dextrose agar (SDA), nutrient broth and nutrient agar media were purchased from Merck (Merck KGaA, Germany). RPMI-1640, fetal bovine serum (FBS) and medium 199 were purchased from Sigma (Sigma Chemical Co., St. Louis, MO). Equipment included Agilent 1200 series binary gradient HPLC coupled with diode array detector (Agilent Technologies, Germany); CO₂ incubator (mco-17AIC,Sanyo-Japan); improved neubauer chamber (Marien, Germany). Reference standards employed in the current study, werepurchased from Sigma-Aldrich USA, include droxithromycin; cefixime; terbinafine; doxorubicin; surfactin; amphotericin-B; 5-Florouracil and vincristine.

The study involved the use of analytical grade solvents which were purchased from Sigma (Sigma-Aldrich USA) i.e. n-hexane (n-Hex); Chloroform (CHCl₃); Acetone (Ace); Ethyl acetate (EtOAc); Ethanol ((EtOH); MEthanol (MeOH) and Dimethyl sulfoxide (DMSO). The reagents; Gallic acid (G7385); Quercetin (Q4951); 2,2-diphenyl-1-picryl-hydrazyl (Cat#757621; DPPH); Potassium acetate; Aluminum Chloride; Folin-ciocaltau (F-C) reagent -Sodium carbonate; Ascorbic acid (A5960); Ammonium molybdate; Sodium phosphate; Sulfuric acid; Ferric cyanide; Trichloroacetic acid and Potassium ferricyanide were procured from Merck (Merck KGaA, Germany). Roxithromycin (R4393); Cefixime (1097658); Terbinafine (T8826), Doxorubicin (D1515), Taxol (T1912) and Amphotericin-B (A2942) was obtained from Sigma (Sigma-Aldrich USA) were used Reference standards in the current study.