The largest database of trusted experimental protocols

2 protocols using ab50517

1

Antibody-based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were used against the following proteins: Sirt1 (ab50517), Sirt5 (ab195436), ERRα (ab76228), ERRβ (ab19331), α-SMA (ab5694), collagen 1A1 (ab34710, Abcam); Sirt2 (sc-28298), SHP (sc-271511), and CYP7A1 (sc-518007, Santa Cruz Biotechnology); Sirt4 (3224) and Sirt7 (3099, Biovision); Sirt3 (5490s), Sirt6 (12486s), Ac-lysine (Ac-K) (9441s), p-STAT3 (Y705) (9145s), STAT3 (12640s), Bax (2772s), cleaved caspase-3 (9664s), p-ERK (4376s), ERK (4695s), p-JNK (4671s), JNK (9258s), p-p38 (9211s), p38 (9212s), and p-JAK2 (4406s; Cell Signaling Technology); Bcl2 (BS1511) and GAPDH (AP0066, Bioworld Technology); ERRγ (PP-H6812-00, Perseus Proteomics); or HSP90 (ADI-SPA-836-F, Enzo Life Sciences).
+ Open protocol
+ Expand
2

Molecular Mechanisms of Cellular Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
CAY10591 and SRT1720 were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Primary antibodies against β-actin, CREB (sc-25785), and NF-κΒ p65 (sc-7151) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against phospho-AMPKThr172 (#2535), acetyl-p53Lys379 (#2570), phospho-p53Ser15, phospho-mTORSer2448, and phospho-CREBSer133 (#9198) were purchased from Cell Signaling Technology. The primary antibodies against iNOS (610431) and p53 were purchased from BD Transduction Lab (Lexington, KY, USA). The primary antibody against COX-2 (aa 570-598) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Antibodies against α-tubulin (T5168), acetylated tubulinLys40 (T7451), HDAC6, EX527 (E7034), and TSA (T8552) were purchased from Sigma Aldrich (St. Louis, MO). Antibodies against HO-1 were purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). Antibodies against β-actin (ab6267) and SIRT1 (ab50517) were purchased from Abcam (Cambridge, MA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!