The human CRC cell lines HCT116 and SW620 were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. HCT116 and SW620 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin. The HCT116 (1×104 cells/well) and SW620 (2×104 cells/well) cells were pretreated with JNK inhibitor (SP600125, 10 µM; Beyotime Institute of Biotechnology) and p38 inhibitor (SB203580, 10 µM; Beyotime Institute of Biotechnology) for 2 h, and then treated with 250 µg/ml RPMTG for 24 h in a 37°C incubator containing 5% CO2. In subsequent experiments, the effects of RPMTG combined with JNK inhibitors or p38 inhibitors on proliferation and apoptosis were evaluated.
Sw620
Manufactured by Beyotime
Sourced in China
The SW620 is a cell line derived from a lymph node metastasis of a colon adenocarcinoma. It is commonly used in cancer research for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Sw620, by National Collection of Authenticated Cell Cultures (2 mentions)
Hct116, by National Collection of Authenticated Cell Cultures (2 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Sw620, by Thermo Fisher Scientific (1 mentions)
Hct116, by Beyotime (1 mentions)
Sp600125, by Beyotime (1 mentions)
Sb203580, by Beyotime (1 mentions)
Sw480, by National Collection of Authenticated Cell Cultures (1 mentions)
Dld 1, by National Collection of Authenticated Cell Cultures (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Penicillin, by Beyotime (1 mentions)
Hct116, by Keygen Biotech (1 mentions)
Dld 1, by Beyotime (1 mentions)
Sw480, by Beyotime (1 mentions)
Mccoy s 5a medium, by Keygen Biotech (1 mentions)
2 protocols using sw620
1
Evaluating RPMTG's Effects on CRC Cell Lines
2
Culturing Colorectal Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human normal colonic epithelial cell line FHC and CRC cell lines HCT116, SW480, DLD1, LoVo, HCT8, SW620 and HT29 were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China). HCT116 and HT29 cells were cultured in McCoy’s 5 A medium (KeyGEN BioTECH, China) with 10% FBS (Gibco, USA), 1% penicillin and streptomycin (Beyotime, China) while FHC, SW480, DLD1, LoVo and HCT8 cells were cultured in RPMI 1640 medium and SW620 cells were cultured in L15 medium. All cells were cultured at 37℃ in a humidified incubator with 5% CO2.
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