G box digital developer

Western blotting was carried out using a G‐Box digital developer (Syngene, Cambridge, UK) to develop chemiluminescent blots and a LI‐COR^® Odyssey for infrared imaging. Densitometry for chemiluminescent blots was carried out on ImageJ^®. The LI‐COR^® Odyssey Infrared Imaging System (version 3.0.30) was used to quantify bands for all quantitative DISC IPs.

Western blotting was carried out as previously described^{19 (link)}. Nuclear/Cytoplasmic fractionations are described in detail in Supplementary methods 1. cIAP1- and cIAP2-specific antibodies were from Enzo (Exeter, UK). XIAP, RIPK1, Acetylated-Lysine, HDAC1- and caspase 3-specific antibodies were from Cell Signaling Technology (Danvers, MA, USA). Caspase 8 antibody was from Alexis Biochemicals (San Diego, CA, USA). FLIP antibody was from Adipogen (San Diego, CA, USA). PARP antibody was from eBioscience (San Diego, CA). FADD antibody was obtained from BD Transduction Laboratories (Franklin Lakes, NJ, USA). Ku70 and HSP90 antibodies were from Santa Cruz Biotechnology (Dallas, Texas, USA). Secondary horseradish peroxidase-conjugated antibodies (Amersham, Buckinghamshire, UK) were used for detection on a G-Box digital developer (Syngene. Cambridge, UK).