L-Proline (>99%), PEG 6000, ninhydrin, 2,2′-azobis(2 methylpropionamidine)dihydrochloride (AAPH), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and potassium ferricyanide (K3[Fe(CN)6]) were acquired from Acros Organics (Geel, Germany). Lactic acid, and fluorescein were purchased from Panreac (Barcelona, Spain). Sodium carbonate anhydrous (Na2CO3), ferric chloride (FeCl3), Folin-Ciocalteu’s reagent, and gallic acid were acquired from VWR (Leuven, Belgium). Ascorbic acid, acetic acid, and potassium iodide were acquired from Merck (Darmstadt, Germany). Salicylic acid (SA), methyl jasmonate (MeJA), Fe3O4 nanoparticles (50–100 nm), hydrogen peroxide (H2O2), trichloroacetic acid (TCA), L- proline (≥99%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3,4-dihydroxy-L-phenylalanine (L-DOPA), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt tablets (ABTS), mushroom tyrosinase (EC 1.14.18.1), kojic acid, potassium persulfate (K2S2O8), potassium bromide, HPLC-MS-grade water, HPLC-MS-grade acetonitrile, luteolin, epigallocatechin gallate, protocatechuic acid, and formic acid were obtained from Sigma–Aldrich (Steinheim, Germany). Rosmarinic acid and quercetin were supplied by Extrasynthese (Genay, France), and ρ-coumaric, caffeic acid, and catechin were provided by AASC Ltd. (Southhampton, UK).
6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
(±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) is a water-soluble vitamin E analog that functions as an antioxidant.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Merck Group (3 mentions)
Protocatechuic acid, by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Rosmarinic acid, by Extrasynthese (3 mentions)
Lactic acid, by ITW Reagents (3 mentions)
L proline, by Merck Group (2 mentions)
Kojic acid, by Merck Group (2 mentions)
Ninhydrin, by Thermo Fisher Scientific (2 mentions)
Potassium iodide, by Merck Group (2 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride, by Thermo Fisher Scientific (2 mentions)
Hplc ms grade water, by Merck Group (2 mentions)
Hplc ms grade acetonitrile, by Merck Group (2 mentions)
L proline, by Thermo Fisher Scientific (2 mentions)
Luteolin, by Merck Group (2 mentions)
Epigallocatechin gallate (egcg), by Merck Group (2 mentions)
Quercetin, by Extrasynthese (2 mentions)
Formic acid, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Gallic acid, by Avantor (2 mentions)
6 protocols using 6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox
1
Comprehensive Reagent Acquisition for Research
2
Comprehensive Reagent Procurement for Analytical Experiments
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L-Proline (>85%), ninhydrin, choline chloride, and (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) were purchased from Acros Organics (Geel, Germany). Lactic acid was acquired from Panreac (Barcelona, Spain), and gallic acid, Folin–Ciocalteu (F-C) reagent, and sodium carbonate from VWR (Leuven, Belgium). Sodium hydroxide (NaOH), ascorbic acid, potassium iodide, and acetic acid were supplied by Merck (Darmstadt, Germany). Trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), hydrogen peroxide (H2O2), L-Proline (≥99%), indole-3-acetic acid, toluene, Bradford reagent, glucose, shikimic acid, periodic acid, cinnamic acid, Bovine Serum Albumin (BSA), luteolin, protocatechuic acid, and epigallocatechin gallate, HPLC-MS-grade water, HPLC-MS grade acetonitrile, and formic acid were purchased from Sigma–Aldrich (Steinheim, Germany). Quercetin and rosmarinic acid were acquired from Extrasynthese (Genay, France). Catechin, caffeic, and ρ-coumaric acids were supplied by AASC Ltd. (Southhampton, UK). Phenol, glycine, sulfuric acid, 6-benzyladenine were acquired from Fluka (Buchs, Switzerland), and L-phenylalanine was obtained from Fisher Scientific (Leicestershire, UK).
3
Antioxidant and Enzyme Inhibition Assays
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Sodium carbonate (Na2CO3) and Folin–Ciocalteau reagent were acquired from VWR (Leuven, Belgium). Methanol, ethanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), galanthamine, AChE (Electric-eel, EC 3.1.1.7, Type VIS), acetylthiocholine iodide (ATCI), BChE (EC 3.1.1.8), butyrylthiocholine iodide (BTCI) and 5,5’-dithiobis-[2-nitrobenzoic acid] (Ellman’s reagent or DTNB) were purchased from Sigma-Aldrich (Steinheim, Germany). Fluorescein (FL), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and 2,2-azobis(2-methylpropionamidine) dihydrochloride (AAPH) were acquired from Acros Organics (Geel, Germany).
Formic acid, high-performance liquid chromatography (HPLC)-grade acetonitrile, acetic acid and sodium acetate were obtained from Panreac (Barcelona, Spain). Gallic acid, protocatechuic acid, catechin, epicatechin and quercetin 3-O-rutinoside (rutin) were purchased from Sigma-Aldrich (Madrid, Spain) and quercetin 3-O-glucoside and kaempferol 3-O-glucoside from Extrasynthese (Genay, France).
Formic acid, high-performance liquid chromatography (HPLC)-grade acetonitrile, acetic acid and sodium acetate were obtained from Panreac (Barcelona, Spain). Gallic acid, protocatechuic acid, catechin, epicatechin and quercetin 3-O-rutinoside (rutin) were purchased from Sigma-Aldrich (Madrid, Spain) and quercetin 3-O-glucoside and kaempferol 3-O-glucoside from Extrasynthese (Genay, France).
4
Antioxidant and Enzyme Inhibition Assays
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2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) tablets, potassium persulfate (K2S2O8), DPPH, 6-benzyladenine (BA), methanol, trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 3,4-dihydroxy-l -phenylalanine (L-DOPA), acetylthiocholine iodide, S-butyrylthiocholine iodide, galantamine, AChE from Electrophorus electricus (electric eel, EC 3.1.1.7, Type VIS), BChE from equine serum horse-serum (EC 3.1.1.8), tyrosinase (EC 1.14.18.1) from mushroom, kojic acid, CuO nanopowder (<50 nm particle size), ZnO dispersion NPs (<100 nm particle size, ≤40 nm average particle size), and Fe3O4 nanopowder (50–100 nm particle size) were purchased from Sigma-Aldrich (Steinheim, Germany). (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), AAPH and fluorescein were supplied from Acros Organics (Geel, Belgium). Ascorbic acid was acquired from Merck (Darmstadt, Germany). Pure acetone was purchased from José Manuel Gomes dos Santos, Lda (Odivelas, Portugal) and Agar from Duchefa Biochemie B.V. (Haarlem, The Netherlands). Ferulic acid, caffeic acid and apigenin were obtained from AASC Ltd. (Southhampton, UK), and rosmarinic acid was acquired from Extrasynthese (Genay, France).
5
Characterization of Lemon Essential Oil Antioxidant Properties
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Steam-distilled Lemon essential oil was purchased from Green Andina (Bogota, Columbia). Natural waxes were obtained from L&F (Medellín, Columbia). Absolute ethanol (lot SHBK8928), sodium carbonate (Lot 94F-0313), sodium acetate trihydrate (Lot SLBX1773), 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (lot BCBW9015), 2,2-diphenyl-1-picrylhydrazil (lot BCBQ6979), nitroblue tetrazolium (NBT) (lot BCBQ6979), and nicotinamide adenine dinucleotide (NAD) (lot BCBQ6979), were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride.6H 2 O (lot 176057) and dimethyl sulfoxide (lot 183521) were purchased from Fisher Chemical (Sunnyvale, CA, USA). In addition, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox ® ) (97%, lot A0405014) and gallic acid (A0267865) were acquired from Acros Organics (Morris Plains, NJ, USA). Further, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) (lot p31b064) was purchased from Alfa Aesar (Haverhill, MA, USA). Methanol (lot l823009611), Folin-Ciocalteu phenol reagent (lot hc43368401), and tetrahydrofuran (lot dg643), were obtained from Merck (Darmstadt, Germany). 2,2 -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2, 2-diphenyl-1-picrylhydrazil (067k lot 1154), and diammonium salt (lot slbp9592v) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
6
Quantification of Phytochemicals in Foods
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All the chemicals and solvents used were of analytical grade. Oxalic acid, fumaric acid, tartaric acid, malic acid, citric acid, lactic acid, D-(+)-fructose, D-(+)-glucose, sucrose, gallic acid 1hydrate, Folin-Ciocalteu reagent, sodium dihydrogen phosphate, sodium carbonate anhydrous, HPLC-grade acetonitrile and 37% (v/v) chloridric acid were purchased from Panreac Applichem (IWT group, Barcelona, Spain). Methanol and anhydrous di-sodium hydrogen phosphate were supplied by Scharlau Chemie (Scharlab S.L. Barcelona, Spain). Thermostable amyloglucosidase (GOPOD) reagent buffer, GOPOD reagent enzymes, D-(+)-glucose standard solution, amyloglucosidase and pancreatic-α-amylase were purchased from Megazyme International (Ireland). Fluorescein, 2, 2-azobis (2-amidinopropane) dihydrochloride (AAPH) and 6-hydroxy-2, 5, 7, 8-tetramethylchromane-2-carboxylic acid (Trolox) were obtained from Acros Organics (Thermo Fisher Scientific Inc. Waltham, MA, USA).
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