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Uhplc grade methanol

Manufactured by Merck Group
Sourced in Canada, Germany

UHPLC grade methanol is a high-purity solvent used in ultra-high-performance liquid chromatography (UHPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the stringent purity requirements for UHPLC analysis, ensuring consistent and reliable separation performance.

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2 protocols using uhplc grade methanol

1

Artesunate Metabolomics Analysis Protocol

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Plasmodium berghei ANKA strain artesunate (100 g,
batch number: X03511Y123220) was purchased from source leaf organisms
(Shanghai, China). UHPLC grade methanol was provided by Merck (Canada).
Formic acid and acetonitrile were supplied by Thermo Fisher Scientific
(Fair Lawn, NJ, USA). Water was obtained from Watsons (Guangdong,
China).
Vanquish UHPLC (Thermo Scientific, Germany) equipped
with a vanquish autosampler, a vanquish pump, and a vanquish column
compartment; a Q-Exactive Plus Orbitrap MS (Thermo Scientific, Germany),
and an X-30R all-purpose high-speed freeze centrifuge (Berle X-mark,
USA) were used.
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2

UHPLC-based Phytochemical Quantification

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UHPLC-grade methanol, acetonitrile, and formic acid were purchased from Merck (Darmstadt, Germany). The standard compounds rutin trihydrate (89.0% of anhydrous rutin), quercetin (95.0%), quercetin-3-O-glucuronide (Q3OG, 98.1%), chrysin (99%), the enzyme mixes β-glucuronidase/arylsulfatase, and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (Deisenhofen, Germany). Water was purified using a Milli-Q system from Millipore (Bedford, MA, USA). All other chemicals were of analytical reagent grade.
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