Anti cleaved caspase 8 antibody clone 12f5

Manufactured by Enzo Life Sciences

The Anti-cleaved caspase-8 antibody clone 12F5 is a laboratory reagent used to detect the cleaved form of caspase-8 protein. Caspase-8 is an enzyme involved in the apoptosis (programmed cell death) pathway. This antibody can be used in various analytical techniques to study apoptosis-related processes.

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Lab products found in correlation

Anti trail antibody clone iii6f, by Enzo Life Sciences (2 mentions) Mach4 universal hrp polymer detection system, by Biocare Medical (1 mentions) Anti cleaved caspase3 antibody clone 5a1e, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Caspase-8 (22 citations) Anti-caspase-8 (6 citations) Caspase-8 antibody (2 citations)

2 protocols using anti cleaved caspase 8 antibody clone 12f5

Apoptosis Signaling Pathway Analysis

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Cells were washed with PBS, centrifuged and re-suspended in PBS to create cytopreparations on glass slides, which were exposed to the following primary antibodies: anti-FAS/CD95 antibody clone DX2 (Enzo Life Sciences, Farmingdale, NY), anti-FASL antibody clone G247-4 (BD Pharmingen San Jose, CA), anti-cleaved caspase-3 antibody clone 5A1E (Cell Signaling), anti-DR4 polyclonal antibody sc-6823 (Santa Cruz Biotechnology, Dallas, TX), anti-TRAIL antibody clone III6F (Enzo Life Sciences), anti-cleaved caspase-8 antibody clone 12F5 (Enzo Life Sciences), and anti-cleaved caspase-9 antibody clone Asp330 (Cell Signaling). Proteins were visualized using the MACH4 Universal HRP-Polymer detection system (Biocare Medical, Concord, CA) and 3,3-diaminobenzidine (DAB). All slides were counterstained with methylene blue (MB).

Salva K.A, & Wood G.S. (2015). Epigenetically Enhanced Photodynamic Therapy (ePDT) is Superior to Conventional Photodynamic Therapy for Inducing Apoptosis in Cutaneous T-Cell Lymphoma. Photochemistry and photobiology, 91(6), 1444-1451.

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Comprehensive Immunohistochemical Profiling of Cell Death Pathways

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After collection and washing with PBS, the cells were centrifuged and re-suspended in PBS to produce cytopreparations on positively charged microscope glass slides. The following panel of primary antibodies was used for biomarker detection: anti-FAS/CD95 antibody clone APO-1-1 (Enzo Life Sciences, Farmingdale, NY), anti-FASL antibody clone 5G51 (Enzo Life Sciences), anti-TRAIL antibody clone III6F (Enzo Life Sciences), anti-DR4 antibody clone DJR1 (Thermo Fisher Scientific, Waltham, MA), anti-DR5 polyclonal antibody (Novus Biologicals, Littleton, CO), anti-TNF-R1 antibody clone H398 (Enzo Life Sciences), anti-TNFα antibody clone 2C8 (Abcam, Cambridge, MA), anti-cleaved caspase-3 antibody clone 31A1067 (Enzo Life Sciences), anti-cleaved caspase-8 antibody clone 12F5 (Enzo Life Sciences), anti-cleaved caspase-9 polyclonal antibody (Abcam). Methylene blue (MB) was applied to all slides as counterstain. Protein visualization was performed with the MACH4 Universal HRP-Polymer detection assay (Biocare Medical, Concord, CA) and 3,3-diaminobenzidine (DAB).

Salva K.A., Kim Y.H., Rahbar Z, & Wood G.S. (2018). Epigenetically Enhanced PDT (ePDT) Induces Significantly Higher Levels of Multiple Extrinsic Pathway Apoptotic Factors than Standard PDT, Resulting in Greater Extrinsic and Overall Apoptosis of CTCL. Photochemistry and photobiology, 94(5), 1058-1065.

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