Irinotecan cpt 11

Cyclohexane, hexanol, triton X-100, toluene, TEOS, APTMS, and ICG were purchased from Sigma (St. Louis, MO, USA). DPPC and cholesterol were purchased from Anatrace (Maumee, OH, USA). DOX was purchased from LC laboratories (Woburn, MA, USA). Irinotecan (CPT-11) was purchased from Selleck Chemicals (Houston, TX, USA). The CCK-8 cell proliferation reagent was purchased from Dojindo Molecular Technologies (Rockville, MD, USA). Fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Invitrogen (Carlsbad, CA, USA). The RPMI 1640 and EMEM cell culture media were purchased from ATCC (Manassas, VA, USA). All other chemicals were purchased from Sigma unless specifically mentioned otherwise.

The human colon cancer cell lines of HCT116, LoVo, HT29, and HCT15 were obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan). Cells were cultured in RPMI medium containing 7% heat-inactivated fetal bovine serum (Gibco, Carlsbad, CA, USA), 100 units (U)/mL penicillin, and 100 U/mL streptomycin (Gibco). Cell lines were authenticated through a short-tandem repeat-profiling analysis to ensure that no culture contamination had occurred. Verteporfin (VP), 5-fluorouracil (5-FU), and irinotecan (CPT11) were purchased from Selleckchem (Houston, TX, USA) and were dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA). Antibodies against PODXL, TAZ, and phospho-TAZ were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against vimentin, Twist, Slug, and GADPH were obtained from GeneTex (San Antonio, TX, USA). Antibody against phospho-LATS1 was purchased from Cell Signaling Technology (Danvers, MA, USA)