RAW264.7 macrophages were collected and incubated with anti-CD163 antibody (11–1631-80, Thermo, USA) at 4°C in line with the manufacturer’s instructions in dark. Then, cells were analyzed by a flow cytometry system (NovoCyte, ACEA, USA).
Anti cd163 antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-CD163 antibody is a laboratory reagent used in research applications. It is a protein that binds specifically to the CD163 antigen, which is expressed on the surface of certain types of immune cells. The antibody can be used to identify and study these cells in various experimental settings.
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Lab products found in correlation
2 protocols using anti cd163 antibody
1
Quantifying Macrophage CD163 Expression
2
ZIKV RNA Detection and Immune Cell Visualization
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ISH for ZIKV RNA was performed with RNAscope (Advanced Cell Diagnostics) on 5 μm thick slides of formalin fixed, paraffin embedded tissues, according to the manufacturer’s instructions and as described before [25 (link)]. Controls included a positive and negative control probe (ubiquitin C and DapB respectively) and uninfected placenta tissue on which the ZIKV probe was used. For immunohistochemistry, tissues were deparaffinized and rehydrated and boiled for 15 minutes in an EDTA buffer. Tissues were subsequently incubated with anti-cytokeratin-7 antibody (Abcam, ab52870, 1:350 dilution) followed by a HRP labeled goat anti mouse antibody (DAKO 1:100 dilution) to visualize trophoblasts. To visualize Hofbauer cells (HBCs), tissues were incubated with an anti-CD163 antibody (ThermoScientific, 1:100 dilution) followed with a rabbit anti mouse IgG antibody labeled with biotin (DAKO, E03354 1:100 dilution) followed by adding streptavidin-HRP (DAKO, D0397, 1:300 dilution). AEC (3-Amino-9-ethylcarbazole, Abcam) was used as HRP substrate, and counterstaining with Mayer’s hematoxylin (Merck) was performed for all tissues.
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