Prominence lc 20a hplc instrument

Chromatographic analysis was performed using a SHIMADZU Prominence LC-20A HPLC instrument (Shimadzu Corporation, Kyoto, Japan) equipped with a YMC-Pack ODS-AM column (4.6 mm × 250 mm; YMC Co., Ltd., Kyoto, Japan). The detection wavelength was set at 202 nm and the column oven at 25°C. The mobile phase consisted of water (A) and acetonitrile (B). A gradient elution was used as follows: 25% B at 0–5 min, 30–32% B at 5–14 min, 32–38% B at 14–28 min, 38–46% B at 28–30 min, 46–74% B at 30–50 min, 74–80% B at 50–51 min, 80–90% B at 51–60 min, 90–100% B at 60–65 min, and 100–25% B at 65–70 min. The flow rate was kept at 1 mL/min, and the injected volume was 10 μL.

HPLC analysis was performed, the SHIMADZU Prominence LC-20A HPLC instrument (Shimadzu Corporation) and a YMC-ODS Pack column (4.6 mm × 250 mm, YMC Co., Ltd.) was used. The parameters of the HPLC were conducted according to the methods of Xue Peng.^{16 (link)} The detection wavelength was set at 202 nm and the column oven was maintained at 25 °C. Solvent A was water and solvent B was acetonitrile. The linear UPLC gradient elution programs were used as follows: 5 min 10% B; 10 min 15% B; 15 min 20% B; 35 min 28% B; 50 min 40% B; 60 min 60% B; 70 min 70% B and 75 min 10% B. The separation was operated at a flow rate was of 1 mL min⁻¹ and the injection volume was 10 μL.