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3 protocols using bay k 8644

1

Pharmacological Agents for Biological Assays

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Serotonin (purity ≥98%), prostaglandin F (purity ≥99%), phenylephrine hydrochloride (purity ≥99%), l‐homocysteine (purity ≥98%), nicardipine (purity ≥98%), barium chloride (purity ≥99.9%), tetraethylammonium (TEA; purity ≥99.5%), 4‐aminopyridine (4‐AP; purity ≥99%) and glibenclamide (purity ≥ 99) were purchased from Sigma Aldrich (St. Louis, MO, USA). Minoxidil (purity ≥99%) was generously donated from the pharmaceutical company Bosnalijek (Sarajevo, Bosnia and Herzegovina), and (±)BAY K 8644 (purity ≥97.5%) was purchased from Cayman Chemical Company. Their stock solutions were prepared either in distilled water or in 99.9% ethanol (with the exception of minoxidil, for which a stock solution was prepared in propylene glycol), according to their solubility, and the subsequent dilutions were carried out in distilled water. The final concentrations of solvent never exceeded 0.33% in the 10 mL organ bath. All solutions were kept at 0–4°C when not used in experiments.
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2

Preparation of Krebs-Ringer Bicarbonate Solutions

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Potassium chloride (KCl), sodium chloride (NaCl), magnesium sulphate (MgSO 4 ), calcium chloride (CaCl 2 ), glucose (C 6 H 12 O 6 ), potassium phosphate (KH 2 PO 4 ), sodium carbonate (NaHCO 3 ), barium chloride (BaCl 2 ), and ethylenediaminetetraacetic acid (EDTA) were obtained from Lach-Ner s.r.o. (Neratovice, Czech Republic) and used to prepare Krebs-Ringer bicarbonate solution and Ca 2+ -free Krebs-Ringer bicarbonate solution.
Other substances: serotonin (5-HT; purity ≥98%), nicardipine (NCP; purity ≥98%), CRV (purity ≥98%), N (gamma)-nitrol-arginine methyl ester (L-NAME; purity ≥98%), tetraethylammonium (TEA; purity ≥99.5%), 4-aminopyridine (4-AP; pu-rity ≥99%), glibenclamide (GLB; purity ≥ 99%), and isopentenyl pyrophosphate (IPP; purity ≥95%) were purchased from Sigma Aldrich (St. Louis, MO, USA), while (±) BAY K 8644 (purity ≥97.5%) was purchased from Cayman Chemical Company. Their stock solutions were prepared either in distilled water or in 99.9% ethanol, according to their solubility, and the subsequent dilutions were carried out in distilled water. All solutions were kept at 0-4 • C when not used in experiments.
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3

Molecular Profiling of Cell Signaling

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Sclareol (CAS: 515-03-7, 98%), oxytocin, sodium bicarbonate, carbachol, acetylcholine (Ach), mannitol, glucose, potassium chloride, potassium phosphate, magnesium sulfate, calcium chloride, estradiol, and dimethyl sulfoxide (DMSO) were purchased from (Sigma-Aldrich, St. Louis, MO, USA). PGF and Bay K 8644 were purchased from (Cayman Chemical Company, Ann Arbor, MI, USA).
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