Ethacrynic acid ea

Ethacrynic acid (EA; Sigma-Aldrich, Saint Louis, MO, USA) was reconstituted in dimethyl sulfoxide (DMSO) to a stock solution of 64 mM. Fluorochromes used for flow cytometry analysis were purchased from Life Technologies (ThermoFisher; Carlsbad, CA, USA) and reconstituted in DMSO, except for propidium iodide (PI) and peanut agglutinin conjugated with fluorescein isothiocyanate (PNA-FITC), which were diluted in phosphate-buffered saline 1× (PBS). The antibody against GSTM3 (ref. ARP53561_P050), as well as its specific blocking peptide (ref. AAP53561), were purchased from Aviva Systems Biology (San Diego, CA, USA). Secondary antibodies for immunoblotting analysis were goat anti-rabbit and rabbit anti-mouse conjugated with horseradish peroxidase (ref. P0448 and ref. P0260; Dako, Derkman A/S; Denmark, respectively), whereas that for immunofluorescence analysis was an anti-rabbit antibody conjugated with Alexa Fluor 488 (ref. A32731; ThermoFisher).

Venetoclax, AZD-4320, Navitoclax, ABT-737, AZD-5991, 5-Azacytidine (5-Aza), Etoposide and MK-571 were purchased from MedChemExpress (USA). Midostaurin, Gilteritinib, Ivosidenib and Enasidenib were acquired from Selleck Chemicals (USA). Reversan was obtained from Merck (Germany). Ethacrynic acid (EA) was purchased from Sigma-Aldrich (USA). All compounds were diluted in DMSO. Buthionine sulfoximine (BSO) was purchased from Merck (Germany) and diluted in ddH₂O. For in vivo experiments, AZD-4320 was dissolved in 30% 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD, Merck (Germany) solution in ddH₂O (3 mM stock).