Microstation reader

Manufactured by Biolog

Sourced in United States

The Biolog Microstation™ reader is a compact and versatile laboratory instrument designed for microbial identification and metabolic profiling. It functions by measuring the respiratory activity of microorganisms in response to various carbon sources and chemical reagents, providing data for analysis and interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Geniii microplates, by Biolog (1 mentions) Ecoplate, by Biolog (1 mentions) Phenotype microarray, by Biolog (1 mentions) Redox dye mix a, by Biolog (1 mentions) Gen 3 microplate, by Biolog (1 mentions) 96 well microplate, by Biolog (1 mentions) Bug agar, by Biolog (1 mentions)

Spelling variants of this product

Biolog MicroStation (22 citations) MicroStation 2 Reader (2 citations)

6 protocols using microstation reader

Bacterial Identification Using Biolog System

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Biolog (Biolog Inc., Hayward, CA, USA; http://www.biolog.com/microID.html) utilizes and identification test panel (YT Micro Plate) consisting of a matrix of 8–12 wells (Fig. 3). The first three rows contain 35 carbon source oxidation tests using tetrazolium violet as an indicator of oxidation. The next five rows contain carbon assimilation tests which are scored turbidometrically against a negative control panel containing only water. The last row contains two carbon sources and tests for the co- utilization of various carbon sources with D-xylose. The hardware (Biolog MicroStation Reader) consists of an automated plate reader coupled with a computer, which interprets the results and compares them with the resident database which currently includes 267 species.
The biochemical composition of bacterial isolates was determined by standard determinative tests as outlined in the BACTID system, in which pre-selected media were incubated after inoculation with single bacterial colonies (Fig. 4).

Al-Dhabaan F.A, & Bakhali A.H. (2016). Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community. Saudi Journal of Biological Sciences, 24(4), 901-906.

+ Open protocol

+ Expand

Biolog GEN III MicroPlate Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Potential carbon-source utilization and the degree of sensitivity to chemicals of the isolates were assessed using BIOLOG GEN III MicroPlates according to the manufacturer’s instructions. The reactions were observed after 24 h incubation at 30 °C and read using an automated Biolog MicroStation Reader.

Shi Y., Yuan Y., Feng Y., Zhang Y, & Fan Y. (2023). Bacterial Diversity Analysis and Screening for ACC Deaminase-Producing Strains in Moss-Covered Soil at Different Altitudes in Tianshan Mountains—A Case Study of Glacier No. 1. Microorganisms, 11(6), 1521.

+ Open protocol

+ Expand

Soil Microbial Functional Diversity Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Functional diversity of the soil microbial community was assessed using Biolog Eco Plates^™ as described by Garau et al. (2007). The plate was composed of 96 wells containing a triplicate set of 31 carbon sources (ten carbohydrates, seven carboxylic acid (CA), four polymers, six amino acids, two phenolic compounds, and two amines) as well as three control wells with no carbon. Briefly, approximately 5 g of fresh soil was suspended in 50 mL of saline solution (0.85% NaCl, w/v) in a 250‐mL flask. After being shaken for 30 min (300 rpm) at 25°C, the suspensions were settled for 10 min. Subsequently, each suspension was diluted 100‐fold, and 150 μL of the clear supernatant was inoculated directly into the Biolog plate, which was then incubated in the dark at 25°C for 7 days. Microbial development was monitored by reading the optical density (OD) at 590 nm every 24 h using a Biolog Microstation^™ reader (Biolog., Hayward, CA, USA). The data collected were expressed as the following five parameters: average well color development (AWCD) for the metabolic activity of soil bacterial community, Shannon index (H’), Simpson index (D), substrate evenness (E), and substrate richness (S) at 96 h after addition of sugar alcohols.

Yu H., Si P., Shao W., Qiao X., Yang X., Gao D, & Wang Z. (2016). Response of enzyme activities and microbial communities to soil amendment with sugar alcohols. MicrobiologyOpen, 5(4), 604-615.

+ Open protocol

+ Expand

Phenotypic Characterization of P. ganghwense

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phenotype Microarrays (Biolog, Hayward, CA, USA) were used for the characterization of P. ganghwense C2.2 (DSM 109767) with regard to the usage pf carbon (PM1 plates) and nitrogen (PM3 plates) sources, osmolyte requirements (PM9 plates), and pH tolerance (PM10 plates). Synthetic seawater minimal medium (ASW) [44 (link)] without NH₄Cl was the basal medium for the microarrays, supplemented with: 0.2% (w/v) urea for PM1; 2% (w/v) glycerol for PM3; 0.2% (w/v) urea, 2% (w/v) glycerol, and no NaCl for PM9; and 0.2% (w/v) urea, 2% (w/v) glycerol, 2% (w/v) NaCl, and pH adjusted to 7 before inoculation for PM10. For the PM array inoculation, the strain was grown on ASW supplemented with 1 g/L yeast extract and 5 g/L peptone (designated as “marine peptone”—MP) agar [7 (link)] for 24 h at 20 °C. Transmittance values were adjusted to 65%, and Biolog Redox Dye Mix A (#74221) was added, with a dilution factor of 100. A working volume of 100 µL was used in each well. Plates were incubated at 20 °C, and the optical density at 590 nm (OD₅₉₀) was measured at 24 h intervals using the Biolog Microstation Reader (Biolog, Hayward, CA, USA). For data analysis, the OD₅₉₀ values measured at the time of inoculation were subtracted from each of the following readings. Heat maps were generated using a custom python script.

Lascu I., Tănase A.M., Jablonski P., Chiciudean I., Preda M.I., Avramescu S., Irgum K, & Stoica I. (2022). Revealing the Phenotypic and Genomic Background for PHA Production from Rapeseed-Biodiesel Crude Glycerol Using Photobacterium ganghwense C2.2. International Journal of Molecular Sciences, 23(22), 13754.

+ Open protocol

+ Expand

Biolog Phenotypic Characterization of Pseudomads

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Biolog (Biolog, USA) system with GEN III microplate was used for the phenotypic characterization of the Psa isolates (S1 Table) and other strains of the Pseudomonad species complex (S6 Table). All strains were grown in KB medium (agar 1.2%) for 48 hours. Fresh colonies, isolated from pure cultures, were chosen to prepare the Biolog inoculum in the Inoculating Fluid following the manufacturer protocol [51 , 52 ]. The turbidity was adjusted to 90% as recommended for default protocol and 100μL of the inoculum was distributed in each well in the Biolog 96-well microplate. The plates were kept at 28°C for 7 days, and daily records were made in a Biolog MicroStation Reader (Biolog, USA). The experiment was independently replicated (two times) following the same method. Among the replicates for each well tested, results classified as borderline and negative means negative, and borderline with positive means positive. Results were recorded and analysed with the R software using the FactoMineR package for PCA and hierarchical classification analysis.

Mariz-Ponte N., Gimranov E., Rego R., Moura L., Santos C, & Tavares F. (2022). Distinct phenotypic behaviours within a clonal population of Pseudomonas syringae pv. actinidiae. PLoS ONE, 17(6), e0269343.

+ Open protocol

+ Expand

Automated Microbial Identification using Biolog MicroStation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Biolog MicroStation System and GEN III microplate (Biolog Inc., Hayward, CA, United States) is an automated microbial identification system based on aerobic metabolic activities. The GEN III plate contains 95 different carbon substrates based on interpreting patterns of sole carbon substrate utilization indicated by color development in a 96-well microtiter plate. By analyzing the similarity of the metabolic fingerprints between test strains and standard strains in the kinetic database by Biolog software, the strains are identified. In this study, the strains M13, M15, and M17 were first cultured on BUG agar (provided by Biolog) and inoculated into a GEN III plate. After being cultured at 30°C for 24 h, the plate was read by a Biolog MicroStation reader to generate strain identification (Wozniak et al., 2019 (link)).

Wu J., Chen Y., Xu X., Ren W., Zhang X., Cai X., Huang A., Zeng Y., Long H, & Xie Z. (2022). Screening of bioflocculant and cellulase-producing bacteria strains for biofloc culture systems with fiber-rich carbon source. Frontiers in Microbiology, 13, 969664.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!