Emc005

Manufactured by Neobioscience

Sourced in China, United States

The EMC005 is a laboratory equipment designed for electrochemical measurements. It features precision instrumentation for accurate and reliable data collection during electrochemical experiments.

Automatically generated - may contain errors

Lab products found in correlation

Emc004, by Neobioscience (3 mentions) Emc102a, by Neobioscience (3 mentions) Emc001b, by Neobioscience (2 mentions) Emc102a 96, by Neobioscience (1 mentions) Emc003, by Neobioscience (1 mentions) Emc101g 96, by Neobioscience (1 mentions) Biotin anti mouse ige, by BD (1 mentions) Elx800, by Agilent Technologies (1 mentions) Emc016, by Neobioscience (1 mentions)

5 protocols using emc005

Cytokine Level Determination Protocol

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The level of TNF-α, IL-6, and IL-10 was determined by commercial kit (NEOBIOSCIENCE, EMC102a.96, EMC004.96, and EMC005.96) according to the manufacturer's protocol.

Wang F., Cen Z., Liu Z., Gan J., Zhang X., Cui Q., Gong S., Chang P, & Chen P. (2021). High-Fat Diet-Induced Fatty Liver Is Associated with Immunosuppressive Response during Sepsis in Mice. Oxidative Medicine and Cellular Longevity, 2021, 5833857.

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Measuring Cytokine and IgE Levels

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Cytokine concentrations in BALF were measured with commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions. ELISA kits were used to measure interferon (IFN)-γ (EMC101g.96, NeoBioscience, Shenzhen, China), interleukin (IL)-4 (EMC003.96, NeoBioscience, Shenzhen, China), IL-5 (EMC108.96, NeoBioscience, Shenzhen, China), IL-13 (EMC124.96, NeoBioscience, Shenzhen, China), IL-10 (EMC005.96, Neo-Bioscience, Shenzhen, China) and transforming growth factor (TGF)-β (EMC107b.96, NeoBioscience, Shenzhen, China). OVAspecific IgE was assessed by ELISA (439807, BioLegend, San Diego, CA). HDM-specific IgE expression was assessed using a modified protocol. 19 In brief, HDM was coated onto 96-well plates (2.5 μg/well) and incubated overnight at 4 °C. After blocking with 5% FBS in PBS, undiluted serum was added and incubated overnight at 4 °C. After washing, biotin anti-mouse IgE (BD Bioscience-San Jose, CA) was added and incubated at 37 °C for 1 h.

Ding F.X., Liu B., Zou W.J., Li Q.B., Tian D.Y, & Fu Z. (2018). Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the T_reg response in asthma. Pediatric research, 84(1).

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Quantifying Immune Factors in Blood

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Sera were collected from the peripheral blood by centrifugation at 1,000 g. The concentrations of TNF-α, TGF-β, IL-2, IL-10, IFN-β, and IL-6 in peripheral blood were measured using ELISA kits according to the manufacturers' instructions [EMC102a, EMC002, EMC005, EMC107b, EMC004, EMC016 (NeoBioscience)]. Optical densities were measured at 450 nm using a microplate reader [ELX800 (BioTek)] and a standard curve was drawn to calculate the content of immune factors.

Zhou P., Li Z., Xu D., Wang Y., Bai Q., Feng Y., Su G., Chen P., Wang Y., Liu H., Wang X., Zhang R, & Wang Y. (2019). Cepharanthine Hydrochloride Improves Cisplatin Chemotherapy and Enhances Immunity by Regulating Intestinal Microbes in Mice. Frontiers in Cellular and Infection Microbiology, 9, 225.

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Cytokine Profiling in Cell Supernatants

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Level of IL-1β (EMC001b, Neobioscience), IL-6 (EMC004, Neobioscience), IL-10 (EMC005, Neobioscience) and TNF-α (EMC102a, Neobioscience) in cell supernatants were measured. And CHIL3 levels in serum were measured using an ELISA kit (ELM-YM1, RayBio, GA, USA). According to the manufacturer's instructions. Added samples and standard into strips and incubate for 2.5 hours at room temperature with gentle shaking. Discard the solution and washed. Added 100 µl of prepared biotinylated antibody for 1 hour. Then reacted with prepared Streptavidin solution. TMB One-Step Substrate Reagent used. 45 minutes after chromogenic reaction, stop reaction was added and each well read at 450 nm immediately.

Jin G., Guo N., Liu Y., Zhang L., Chen L., Dong T., Liu W., Zhang X., Jiang Y., Lv G., Zhao F., Liu W., Hei Z., Yang Y, & Ou J. (2023). 5-aminolevulinate and CHIL3/CHI3L1 treatment amid ischemia aids liver metabolism and reduces ischemia-reperfusion injury. Theranostics, 13(14), 4802-4820.

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Inflammatory Cytokine Profiling via ELISA

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The levels of the inflammatory cytokine TNF-α, IL-1β and IL-10 in cell supernatants were measured using ELISA kits (cat. nos. EMC102a, EMC001b and EMC005, respectively; Neobioscience Technology Co., Ltd.) according to the manufacturer’s instructions.

Liao H., Huang J., Liu J., Zhu H., Chen Y., Li X., Wen J, & Yang Q. (2023). Sirt1 regulates microglial activation and inflammation following oxygen-glucose deprivation/reoxygenation injury by targeting the Shh/Gli-1 signaling pathway. Molecular Biology Reports, 50(4), 3317-3327.

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