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Anti rankl

Manufactured by Cell Signaling Technology
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Anti‐RANKL is a laboratory product that targets the RANKL protein. RANKL is a key regulator of bone remodeling and osteoclast differentiation. Anti‐RANKL can be used to detect and study the RANKL protein in research applications.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (1 mentions) Phalloidin conjugate solution, by Merck Group (1 mentions) Trap solution, by Merck Group (1 mentions) Anti tnf α, by Cell Signaling Technology (1 mentions) Anti opg, by Cell Signaling Technology (1 mentions) Goat anti rabbit horseradish peroxidase conjugate, by Bio-Rad (1 mentions) Alamarblue reagent, by Merck Group (1 mentions) Anti tgf β, by Cell Signaling Technology (1 mentions) Elisa kit, by Merck Group (1 mentions) Anti il 6, by Cell Signaling Technology (1 mentions) Anti il 1β, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Faststart essential dna green master, by Roche (1 mentions) Surebeads protein a magnetic beads, by Bio-Rad (1 mentions) Rabbit anti opg, by Abcam (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Rankl, by R&D Systems (1 mentions)

2 protocols using anti rankl

1

Osteoclast Differentiation and Activation Assay

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α‐Minimal essential medium (α‐MEM), penicillin, streptomycin, foetal bovine serum (FBS), qPCR SuperMix UDG kit were purchased from Invitrogen (Carlsbad, CA). Lipopolysaccharides (LPS), macrophage monocyte colony‐stimulating factor (M‐CSF), methylthiazolyldiphenyl‐tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), TRAP solution, Acid Phosphatase Liquicolor Assay kit, Hoechst 33342, ELISA kits, alamar blue reagent and phalloidin conjugate solution were purchased from Sigma‐Aldrich. Anti‐NFATc1 (H‐110), anti‐Ap1 (Sc‐57761) and anti–c‐Fos (H‐125) monoclonal antibodies were purchased from Santa Cruz Biotechnology. Anti–TNF‐α, anti‐RANKL, anti‐OPG, anti–IL‐1β, anti–IL‐6 and anti–TGF‐β were purchased from Cell Signaling Technology. NucleoSpin for RNA extraction kit was purchased from Macherey–Nagel. Toluidine blue stain was purchased from BioGenex. FastStart Essential DNA Green Master was purchased from Roche. Goat anti‐rabbit horseradish peroxidase conjugate was purchased from Bio‐Rad.
Al Mamun M.A., Asim M.M., Sahin M.A, & Al‐Bari M.A. (2020). Flavonoids compounds from Tridax procumbens inhibit osteoclast differentiation by down‐regulating c‐Fos activation. Journal of Cellular and Molecular Medicine, 24(4), 2542-2551.
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2

RANKL Protein Interaction Assay

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8 mm in diameter CGO or MCGO scaffold were incubated with 120 ng of purified RANKL (R&D Systems) in 1 mL of PBS with 1 mM EDTA overnight at room temperature. After washing scaffolds, protein extracts were prepared by morselizing the scaffolds in 500 μL RIPA buffer (Alfa Aesar, Ward Hill, MA). Lysates were then incubated with SureBeads Protein A Magnetic Beads (BioRad, Hercules, CA) with either rabbit anti-OPG (AbCam, Waltham, MA) or anti-RANKL (Cell Signaling Technology, Danvers, MA) at 4 °C overnight. Beads were washed and protein was eluted from beads by resuspending in 40 μL of sodium dodecyl sulfate (SDS) sample buffer and incubating at 70 °C for 10 minutes. The beads were then pelleted and the supernatant was loaded into SDS-PAGE gels for Western Blot analysis.
Price R.N., Cassar C., Brockman A., Duraisingh M., van Vugt M., White N.J., Nosten F, & Krishna S. (1999). The pfmdr1 Gene Is Associated with a Multidrug-Resistant Phenotype in Plasmodium falciparum from the Western Border of Thailand. Antimicrobial Agents and Chemotherapy, 43(12), 2943-2949.
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