Irdye 700 donkey anti rabbit igg

The cell extracts were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose membranes. The membranes were then blocked with Tris-buffered saline and Tween-20 contaning 5% of bovine serum albumin at room temperature for 2 h and incubated with corresponding antibodies overnight at 4 °C. The membranes were washed and incubated with appropriate dilution of IRDye 800 donkey anti-mouse IgG or IRDye 700 donkey anti-rabbit IgG (LI-COR Inc., Lincoln, NA, USA) for 1 h at room temperature. After washing with Tris-buffered saline, the membranes were then analyzed by an Odyssey Infrared Imaging System (LI-COR Inc.). Rabbit anti-poly (ADP-ribose) polymerase antibody, goat anti-CAR antibody, mouse anti-6-his antibody were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Mouse anti-E1A antibody was purchased from Abcam (Cambridge, UK). Goat anti-hIL-3 antibody was purchased from R&D Systems. Rabbit anti-MnSOD antibody was purchased from Epitomics (Burlingame, CA, USA). Rabbit anti-GAPDH antibody, rabbit anti-Bcl-2 antibody, rabbit anti-Bax antibody, rabbit anti-caspase-7 antibody and rabbit anti-caspase-9 antibody were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

The cell extracts were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes. The membranes were then blocked with Tris-buffered saline and Tween 20 contaning 5% of bovine serum albumin at room temperature for 2 h and incubated with corresponding antibodies overnight at 4°C. The membranes were washed and incubated with appropriate dilution of IRDye 800 donkey anti-mouse IgG or IRDye 700 donkey anti-rabbit IgG (LI-COR, Inc., Lincoln, NA, USA) for 1 h at room temperature. After washing with Tris-buffered saline, the membranes were then analyzed by an Odyssey Infrared Imaging System (LI-COR, Inc.).
The rabbit anti-PARP (H250) antibody was purchased from Santa Cruz biotechnology Inc. (Santa Cruz, CA, USA). Rabbit anti-XIAP antibody was purchased from Epitomics (Burlingame, CA, USA). Rabbit anti-GAPDH antibody and rabbit anti-Bcl-2 antibody were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).