Cultured primary microglia were lysed with a buffer containing 1% Triton X-100, 150 mM NaCl, 10 mM NaH2PO4, 15 mM Na2HPO4, 50 mM NaF, 1 mM EDTA, and 1 mM Na3VO4. Cell lysates (500 μg) were incubated with an MJFF2 LRRK2 antibody (5 μL/rxn) and further incubated with agarose-G (20 μL, 50% slurry). After washing three times with lysis buffer, Rac1 (1:3,000, Millipore) and LRRK2 (1/1,000, Millipore) were used to detect the levels of protein bound to rat LRRK2 by Western Blot.
Lrrk2
Manufactured by Merck Group
LRRK2 is a laboratory equipment product developed by Merck Group. It is a specialized device designed for research and scientific applications. The core function of LRRK2 is to facilitate the study and analysis of the LRRK2 protein, which is associated with certain neurodegenerative disorders.
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Lab products found in correlation
1 protocol using lrrk2
1
LRRK2 Protein Interaction Assay
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