Neaa nonessential amino acids

Manufactured by Thermo Fisher Scientific

NEAA (nonessential amino acids) is a laboratory product that provides a source of nonessential amino acids, which are required for various cellular processes. It is a mixture of amino acids that are not considered essential for human health, but are still necessary for the proper functioning of cells and organisms.

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2 protocols using neaa nonessential amino acids

Cultivation of Human Cell Lines

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U-2 OS cells (obtained from ATCC) were cultivated in RPMI 1640 medium supplemented with 10% FBS (Life Technologies), sodium pyruvate, 2 mM glutamine, penicillin and streptomycin (Sigma), 20 mM HEPES, and glucose (4 mg/ml). NPC1 fibroblasts (obtained from Coriell Repository) were maintained in EMEM medium supplemented with NEAA (nonessential amino acids) (Life Technologies), 2 mM glutamine, penicillin and streptomycin (Sigma), and 15% FCS. HDFa fibroblasts were grown in Medium 106 with Low Serum Growth Supplement (LSGS) as recommended by manufacturer (Life Technologies).

Králová J., Jurášek M., Krčová L., Dolenský B., Novotný I., Dušek M., Rottnerová Z., Kahle M., Drašar P., Bartůněk P, & Král V. (2018). Heterocyclic sterol probes for live monitoring of sterol trafficking and lysosomal storage disorders. Scientific Reports, 8, 14428.

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Culturing Human Stem Cells for Regenerative Medicine

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Human mesenchymal stem cells (hMSCs) (Institute for Regenerative Medicine, Texas A&M University) were cultured in growth medium (high glucose supplemented with 10% (vol/vol) FBS, 100 units/mL of penicillin, and 100 μg/mL of streptomycin). The hMSCs were trypsinized upon reaching 70% confluence and passage 5 cells were used for the experiments. Human induced pluripotent stem cells (hiPSCs) were expanded on mitomycin C-treated mouse embryonic fibroblast (MEF) feeder cells with Knockout DMEM (Life Technologies) containing 10% knockout serum replacement (Life Technologies), 1% NEAA nonessential amino acids (Life Technologies), 10% human plasmanate (Talecris Biotherapeutics), 1% Gluta-MAX (Life Technologies), 55 mM 2-mercaptoethanol (Life Technologies), and 1% penicillin/streptomycin (Life Technologies). 30 ng/mL of bFGF (basic fibroblast growth factor, Life Technologies) was added daily into the growth medium and cells were passaged using Accutase (Millipore) at 80% confluency [49 (link)].

Kar M., Shih Y.R., Velez D.O., Cabrales P, & Varghese S. (2015). Poly(ethylene glycol) hydrogels with cell cleavable groups for autonomous cell delivery. Biomaterials, 77, 186-197.

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