Cd27 bv480

Manufactured by BD

The CD27 BV480 is a fluorescently-labeled monoclonal antibody designed for flow cytometric analysis. It targets the CD27 antigen, which is expressed on various immune cell types.

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Lab products found in correlation

Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Tnf α fitc, by BD (2 mentions) Il 2 bv711, by BD (2 mentions) Il 21 pe, by Thermo Fisher Scientific (2 mentions) Ifn γ pe cy7, by BD (2 mentions) Cd4 apc cy7, by BD (2 mentions) Perforin pe dazzle 594, by BioLegend (2 mentions) Cd69 bv650, by BD (2 mentions) Cd45ro pe cy5, by Beckman Coulter (2 mentions) Cd40l bv605, by BioLegend (2 mentions) Cd8 percp, by BioLegend (2 mentions) Pd 1 bv421, by BioLegend (2 mentions) Pd l1 bv711, by BioLegend (1 mentions) Ki 67 percp cy5, by BD (1 mentions) Cxcr5 alexa fluor 647, by BD (1 mentions) Tigit pe cy7, by BioLegend (1 mentions) Cxcr3 bv605, by BD (1 mentions) Lag3 bv650, by BioLegend (1 mentions) Tim3 percp cy5, by BioLegend (1 mentions) Ccr5 pe dazzle 594, by BioLegend (1 mentions)

2 protocols using cd27 bv480

Multiparameter Flow Cytometry Analysis

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The following fluorochrome-conjugated anti-human mAb were used for flow cytometry studies: LAG3 BV650, TIGIT PE-Cy7, CTLA4 PE, CD25 PE-Dazzle 594, TIM3 PerCP-Cy5.5, CD19 Alexa Fluor 700, CCR4 BV421, ICOS PE-Cy7, CCR5 PE-Dazzle 594, HLA-DR PE, CCR7 FITC, CD38 BV711, PD-L1 BV711, CCR6 Alexa Fluor 700, PD-1 BV421, CD40L BV605, perforin PE-Dazzle 594, and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD4 APC-H7, CD127 BV605, Ki-67 PerCP-Cy5.5, PD-1 BV650, CXCR3 BV605, CD69 BV650, IL-2 BV711, CXCR5 Alexa Fluor 647, IFN-γ PE-Cy7, TNF-α FITC, granzyme B Alexa Fluor 700 and CD27 BV480 from BD Biosciences (San Jose, CA); IL-21 PE from eBioscience, (San Diego, CA); and CD45RO PE-Cy5.5 from Beckman Coulter (Fullerton, CA). LIVE/DEAD Fixable Blue Dead Cell Stain Kit from Thermo Fisher Scientific (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated for optimum concentration before usage.

Rinaldi S., de Armas L., Dominguez-Rodríguez S., Pallikkuth S., Dinh V., Pan L., Gӓrtner K., Pahwa R., Cotugno N., Rojo P., Nastouli E., Klein N., Foster C., De Rossi A., Giaquinto C., Rossi P., Palma P, & Pahwa S. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLoS Pathogens, 17(4), e1009533.

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Multiparametric Flow Cytometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following fluorochrome conjugated anti-human mAb were used for flow cytometry studies: PD-1 BV421, CD40L BV605, Perforin PE-Dazzle 594 and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD69 BV650, IL-2 BV711, CXCR5 Alexa647, IFN-γ PE-Cy7, TNF-α FITC, Granzyme-B and CD27 BV480 from BD Bioscience (San Jose, CA); IL-21 PE from e-Biosciences, (San Diego, CA); and CD45RO-PE-Cy5.5 from Beckman Coulter (Fullerton, CA). Live/Dead® Fixable Blue Dead Cell Stain Kit from ThermoFisher (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated before usage.

Rinaldi S., Pallikkuth S., Cameron M., de Armas L.R., Cotugno N., Pahwa R., Richardson B., Saini S.R., Rocca S., Lain M.G., Williams S.L., Palma P, & Pahwa S. (2019). Impact of early ART initiation on HIV-specific CD4 and CD8 T cell function in perinatally infected children. Journal of immunology (Baltimore, Md. : 1950), 204(3), 540-549.

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