Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich Saint Louis, MO, USA. HPLC Grade Ammonium acetate was purchased from VWR. HPLC-grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) was obtained from Alfa Aesar (Ward Hill, MA, USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. All other chemicals used in this study were of analytical grade. Traut’s reagent (2-Iminothiolane) and Ellman’s reagent were obtained from Pierce (Rockford, IL, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Effendorf centrifuge 5810R, Thermo scientific sorvall RC 6+, Waltham, MA, USA), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
Nhs peg mal 5000
Manufactured by Merck Group
Sourced in United States
NHS-PEG-MAL-5000 is a heterobifunctional crosslinker that consists of a polyethylene glycol (PEG) spacer with an N-hydroxysuccinimide (NHS) ester on one end and a maleimide group on the other end. It is used for the conjugation of proteins, peptides, or other molecules with free amine and thiol groups.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
High performance liquid chromatography (hplc), by Thermo Fisher Scientific (2 mentions)
Minocycline, by Merck Group (2 mentions)
Hplc grade glutaraldehyde, by Thermo Fisher Scientific (2 mentions)
Pd 10 columns sephadex g 25 m, by GE Healthcare (2 mentions)
Milli q plus apparatus, by Merck Group (2 mentions)
Hplc grade ammonium acetate, by Avantor (2 mentions)
Hplc grade water, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Transferrin, by Merck Group (2 mentions)
2 protocols using nhs peg mal 5000
1
Minocycline-Albumin Nanoparticle Synthesis
2
Minocycline-Based Drug Delivery Platform
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Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich U.S.A. HPLC Grade Ammonium acetate was purchased from VWR. HPLC grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) were obtained from Alfa Aesar (USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. Traut's reagent (2-Iminothiolane) and Ellman's reagent was obtained from Pierce (Rockford, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Eppendorf Centrifuge 5810R, Thermo scientific sorvall RC 6+), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
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