HESCs kindly donated by Dr. Haibin Wang (Xiamen University, Xiamen, China) were cultured in Phenol Red-free Dulbecco’s modified Eagle’s medium (DMEM)/F-12 plus 10% charcoal-stripped fetal bovine serum (Biological Industries) at 37 °C in a 5% CO2 atmosphere. To induce decidualization in vitro, HESCs were incubated with 1 μmol/L medroxyprogesterone-17-acetate (MPA) (Sigma-Aldrich, St. Louis, MS, USA) and 0.5 mmol/L N6,20-O-dibutyryladenosine cAMP sodium salt (db-cAMP) (Sigma-Aldrich, St. Louis, MS, USA). The medium was replaced every 2 days.
N6 20 o dibutyryladenosine camp sodium salt db camp
Manufactured by Merck Group
Sourced in United States
N6,20-O-dibutyryladenosine cAMP sodium salt (db-cAMP) is a chemical compound that functions as a cyclic adenosine monophosphate (cAMP) analog. It serves as a tool for research and experimental purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n6 20 o dibutyryladenosine camp sodium salt db camp
1
In vitro Decidualization of HESCs
2
In Vitro Decidualization of HESCs
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HESCs kindly donated by Dr. Haibin Wang (Xiamen University, Xiamen, China) were cultured in Phenol Red-free Dulbecco's modi ed Eagle's medium (DMEM)/F-12 plus 10% charcoal-stripped fetal bovine serum (Biological Industries) at 37°C in a 5% CO 2 atmosphere. To induce decidualization in vitro, HESCs were incubated with 1 μmol/L medroxyprogesterone-17-acetate (MPA) (Sigma-Aldrich, St. Louis, MS, USA) and 0.5 mmol/L N6,20-O-dibutyryladenosine cAMP sodium salt (db-cAMP) (Sigma-Aldrich, St. Louis, MS, USA). The medium was replaced every two days.
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