Western blotting was performed as described previously.21 Anti-MYC monoclonal antibody (Abcam, 1:1000), anti-PCYT1A monoclonal antibody (Cell Signaling, 1:1000), anti-p-AKT monoclonal antibody (Cell Signaling, 1:1000), anti-p-ERK monoclonal antibody (Cell Signaling, 1:1000) and Anti-CDK6 monoclonal antibody (Cell Signaling, 1:1000) were used. Anti-Actin antibody (Cell Signaling, 1:2000) was used to ensure equivalent loading of total protein.
Anti p erk monoclonal antibody
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-p-ERK monoclonal antibody is a research-use only product designed to detect phosphorylated extracellular signal-regulated kinase (p-ERK). It recognizes the active, phosphorylated form of ERK1 and ERK2 proteins. This antibody can be used in various immunodetection techniques to study the activation of the ERK signaling pathway.
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Lab products found in correlation
Anti p akt monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti actin antibody, by Cell Signaling Technology (1 mentions)
Anti myc monoclonal antibody, by Abcam (1 mentions)
Anti akt polyclonal antibody, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by ZSGB-BIO (1 mentions)
Anti gapdh, by ZSGB-BIO (1 mentions)
2 protocols using anti p erk monoclonal antibody
1
Western Blot Analysis of Cellular Proteins
2
Western Blot Antibody Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-NHERF1 monoclonal antibody (1:1000 working dilution) from BD Biosciences (San Jose, CA, USA). Anti-NHERF1 polyclonal antibody, anti-AKT polyclonal antibody, anti-pS473AKT monoclonal antibody, and anti-pERK monoclonal antibody (1:1000 working dilution) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ERK antibody polyclonal antibody (1:1000 working dilution) from Upstate Biotechnology (Lake Placid, NY, USA). Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) monoclonal antibody (1:2000 working dilution) and horseradish peroxidase (HRP)-conjugated secondary antibody were obtained from ZSGB-BIO (Beijing, China).
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