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Anti p erk monoclonal antibody

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-p-ERK monoclonal antibody is a research-use only product designed to detect phosphorylated extracellular signal-regulated kinase (p-ERK). It recognizes the active, phosphorylated form of ERK1 and ERK2 proteins. This antibody can be used in various immunodetection techniques to study the activation of the ERK signaling pathway.

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2 protocols using anti p erk monoclonal antibody

1

Western Blot Analysis of Cellular Proteins

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Western blotting was performed as described previously.21 Anti-MYC monoclonal antibody (Abcam, 1:1000), anti-PCYT1A monoclonal antibody (Cell Signaling, 1:1000), anti-p-AKT monoclonal antibody (Cell Signaling, 1:1000), anti-p-ERK monoclonal antibody (Cell Signaling, 1:1000) and Anti-CDK6 monoclonal antibody (Cell Signaling, 1:1000) were used. Anti-Actin antibody (Cell Signaling, 1:2000) was used to ensure equivalent loading of total protein.
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2

Western Blot Antibody Protocols

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Anti-NHERF1 monoclonal antibody (1:1000 working dilution) from BD Biosciences (San Jose, CA, USA). Anti-NHERF1 polyclonal antibody, anti-AKT polyclonal antibody, anti-pS473AKT monoclonal antibody, and anti-pERK monoclonal antibody (1:1000 working dilution) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ERK antibody polyclonal antibody (1:1000 working dilution) from Upstate Biotechnology (Lake Placid, NY, USA). Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) monoclonal antibody (1:2000 working dilution) and horseradish peroxidase (HRP)-conjugated secondary antibody were obtained from ZSGB-BIO (Beijing, China).
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