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F ab 2 rabbit anti human igm

Manufactured by Jackson ImmunoResearch
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F(ab')2 rabbit anti-human IgM is a laboratory reagent that binds to the Fc portion of human immunoglobulin M (IgM) antibodies. It is produced by enzymatic digestion of rabbit anti-human IgM antibodies, resulting in the F(ab')2 fragment, which retains the antigen-binding capacity but lacks the Fc region.

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Lab products found in correlation

Gardiquimod, by Thermo Fisher Scientific (1 mentions) Cd20 magnetic beads, by Miltenyi Biotec (1 mentions) Gardiquimod, by InvivoGen (1 mentions)

Close spelling variants of this product

Anti-human IgM (9 citations) Rabbit anti-human IgM (3 citations) Human IgM (2 citations)

2 protocols using f ab 2 rabbit anti human igm

1

Purifying and Stimulating B Cells

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B cells were purified from the peripheral blood by positive selection using CD20 magnetic beads (Miltenyi Biotec) and plated for 48 hours at 150,000-200,000 cells per well in a 96-well plate in RPMI containing 10% FBS alone or RPMI+10% FBS and either 2 μg/ml polyclonal F(ab')2 rabbit anti-human IgM (Jackson ImmunoResearch, West Grove, PA), multimeric, soluble, 0.05 μg/ml recombinant-human CD40L (Alexis Biochemicals, San Diego, CA), 2 μg/ml gardiquimod (TLR7 agonist; Invitrogen, Waltham, MA), or 0.5 μg/ml CpG (TLR9 agonist; Invitrogen).
Minto H., Mensah K.A., Reynolds P.R., Meffre E., Rubtsova K, & Gelfand E.W. (2019). A Novel ATM Mutation Associated with Elevated Atypical Lymphocyte Populations, Hyper-IgM, and Cutaneous Granulomas. Clinical immunology (Orlando, Fla.), 200, 55-63.
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2

Purified B Cell Activation Assay

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CD20+ purified B cells were stained for surface markers using fluorochrome labeled antibodies against CD19, CD27, CD10, CD69, CD86, CD25 (Biolegend, San Diego, CA), and CD21 (BD Biosciences). In stimulation experiments, B cells were plated at 150,000–200,000 cells per well in a 96 well plate in RPMI with 10% fetal bovine serum and 2 μg/mL polyclonal F(ab)′2 rabbit anti-human IgM (Jackson Immunoresearch, West Grove, PA), 2 μg/mL Gardiquimod™ (TLR7 agonist; InvivoGen, San Diego, CA), or 0.5 μg/mL CpG (TLR9 agonist; InvivoGen) for 48 hours.
Janssen E., Morbach H., Ullas S., Bannock J.M., Massad C., Menard L., Barlan I., Lefranc G., Su H., Dasouki M., Al-Herz W., Keles S., Chatila T., Geha R.S, & Meffre E. (2014). DOCK8 deficient patients have a breakdown in peripheral B cell tolerance and defective regulatory T cells. The Journal of allergy and clinical immunology, 134(6), 1365-1374.
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