Total plasma cholesterol and triglyceride levels were measured (1489232, Chol CHOD-PAP, Roche, Almere, the Netherlands; 337-B, TG GPO-trinder, Sigma Aldrich, Zwijndrecht, the Netherlands). Measurements were done according to manufacturer's protocols on a Benchmark 550 Micro-plate Reader (170-6750XTU, Bio-Rad, Veenendaal, the Netherlands). For organ lipid analyses, ~50 mg of frozen liver was homogenized for 30 s at 5,000 rpm in a closed tube with 5.0 mm glass beads and 1.0 ml SET buffer (Sucrose 250 mM, EDTA 2 mM, and Tris 10 mM) (35 (link)). Complete cell destruction was done by two freeze-thaw cycles and 3 times passing through a 27- gauge syringe needle and a final freeze-thaw cycle. Protein content was measured with the BCA method (23225, Pierce, Rockford, IL, United States). TG were measured as described above. All analyses were performed according to manufacturers' instructions.
Chol chod pap
Manufactured by Roche
The Chol CHOD-PAP is a laboratory equipment product used for the enzymatic colorimetric determination of cholesterol in biological samples. It measures the concentration of cholesterol in a sample.
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Lab products found in correlation
2 protocols using chol chod pap
1
Plasma and Liver Lipid Analyses
2
Lipoprotein Profile Determination in Mice
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Lipoprotein profiles were determined on plasma samples from mice using an A ¨KTA Basic chromatography system with a Superose 6PC 3.2/30 column (Amersham Biosciences, Roosendaal, the Netherlands) as described previously in detail. 19 After collection, the samples were kept on ice, centrifugated at 4 C for 15 min at 800 rpm and snap frozen in liquid nitrogen. Plasma cholesterol and triglyceride level were measured using a commercially available kit (1489232, Chol CHOD-PAP, Roche, Almere, the Netherlands, 337-B, TG GPO-trinder, Sigma-Aldrich, Zwijndrecht, the Netherlands). Measurements were performed according to manufacturer's protocols on a Benchmark 550 nm micro-plate reader (170-6750XTU, Bio-Rad, Veenendaal, the Netherlands).
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