We assessed the gastrocnemius muscle contractile force in the sham, I/R, and Oxy groups. Under anesthesia with pentobarbital sodium (80 mg/kg, i.p.), the left gastrocnemius muscles were quickly removed and rinsed in ice-cold modified Krebs-Henseleit (K-H) solution saturated by 95% O2 and 5% CO2. The K-H solution contained (in mmol/L) NaCl 118, KCl 4.7, CaCl2 1.8, MgSO4·7H2O 1.2, KH2PO4 1.2, NaHCO3 25, glucose 11, and Hepes 10 (pH 7.4 ± 0.05, gassed with 95% O2 and 5% CO2). The gastrocnemius muscle's proximal end was fixed, while the distal tendon was attached to a mechanical force transducer (AD Instruments, Australia). An initial tension of 1 g was applied to the muscle preparations. The stimuli were delivered through a bipolar electrode placed in a chamber and connected to a stimulator. The gastrocnemius muscle was continuously perfused with K-H solution at 15 ml/min at 37°C for at least 1 h before the experiments. Individual twitch contraction of the gastrocnemius muscle was induced by the stimulation (5 V, 1 Hz, 1 ms pulse), which was repeated 30 times to induce tetanic contractile force (5 V, 120 Hz, 1 ms pulse). The PowerLab (AD Instruments) data acquisition system with LabChart7 software was used to record and analyze the muscle contraction.
Mechanical force transducer
Manufactured by ADInstruments
Sourced in Australia, United Kingdom
The Mechanical Force Transducer is a precision instrument designed to measure mechanical forces. It converts physical force into an electrical signal, allowing for accurate and reliable measurements. The core function of this device is to provide quantitative data on the magnitude and direction of applied forces.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mechanical force transducer
1
Evaluating Gastrocnemius Muscle Contractility
2
Measuring Diaphragm Contractile Force in Mice
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Whole diaphragms from 4-montheold wild-type and dystrophic mice were excised, and contractile force strength was measured following the Treat Neuromuscular Disorders Neuromuscular Network (TREAT-NMD) standard operating procedures (https://treat-nmd.org/researchoverview/preclinical-research/experimental-protocols-fordmd-animal-models, last accessed September 26, 2019) and as previously described. 8 Essentially, diaphragms were placed into Krebs-Ringer solution. Sutures were tied and muscle then attached to an immobile plastic clamp with the central triangular section of the diaphragm being used for testing. Contractile force was measured using a mechanical force transducer (ADInstruments, Oxford, UK), amplifier, and data acquisition setup. Excitation was achieved via local field potentials through platinum electrodes in oxygenated (95% O 2 , 5% CO 2 ) Krebs-Ringer solution, at a constant temperature (37 C). After incremental stretching to establish the optimal excitation-to-force generation length and confirmation of the appropriate voltage twitch stimulus, diaphragm sections were subjected to a 140-V (2-millisecond) stimulus train at 100-Hz frequency for 0.5 to 1 second. The test regimen involved collecting six twitch responses, followed by six tetanic trains, with a 2-minute rest
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