Agar base

To evaluate the microbiological quality, a 10 g sample of muscle was mixed with 90 mL buffered peptone water 0.1% w/w (Microkit BCD046, Madrid, Spain) in a stomacher (IUL Instruments, GmBH, Köningswinter, Germany), inside an aseptic cabinet (Telstar, Bio–II–A. Tarrasa, Spain) and serial dilutions were prepared. Bacterial counts were determined as follows: Total Aerobic were determined by the pour plate method in Plate Count Agar (Oxoid CM0325, Basingstoke, UK) incubated at 30 °C for 42 days [18 ]; Psychrophilic bacteria were analysed in Plate Count Agar (Oxoid CM0325, Basingstoke, UK) and incubated at 4 °C for 10 days [19 ]; Lactic Acid Bacteria were counted on Man Rogosa and Sharpe Agar (Oxoid CM0361, Basingstoke, UK) (30 °C for 2 days) [20 ]^; violet red bile glucose agar (Oxoid CM485, Basingstoke, UK) was used for Enterobacteriaceae incubated at 37 °C for 24 h [21 ]; Total Coliforms were determined using a violet red bile glucose agar (Oxoid CM017, Basingstoke, UK) after incubation at 37 °C for 24 h, Pseudomonas were determined by plate seeding on agar base (Oxoid, Basingstoke, UK) (28 °C-24 h), and Moulds and Yeasts was enumerated with Rose Bengal Agar + Chloramphenicol (Oxoid CM0549, Basingstoke, UK) (25 °C for 5 days) [22 ]. Plating was performed in duplicate, and the results were expressed as log colony-forming unit (CFU) g⁻¹.