Click-iT EdU labeling was conducted using the Click-iT EdU Alexa Fluor 488 Cell Proliferation Kit for Imaging (ThermoFisher) according to manufacturer’s instructions. Following 60-minute incubation with EdU label, cells were washed extensively and the media replaced with EV-free complete cell culture media to allow for TMV shedding. TMVs were isolated as described above, and plated on poly-L-lysine coated coverslips for imaging. Cells and TMVs were fixed and subjected to Click-iT reaction according to manufacturer’s recommended protocol before staining with primary and secondary antibodies as described above.
Click it edu alexa fluor 488 cell proliferation kit for imaging
Manufactured by Thermo Fisher Scientific
The Click-iT EdU Alexa Fluor 488 Cell Proliferation Kit for Imaging is a fluorescent-based assay designed to detect and quantify cell proliferation. It utilizes a thymidine analog, EdU (5-ethynyl-2'-deoxyuridine), which is incorporated into newly synthesized DNA during the S phase of the cell cycle. The incorporated EdU is then detected using a fluorescent Alexa Fluor 488 dye, allowing for the visualization and quantification of proliferating cells.
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2 protocols using click it edu alexa fluor 488 cell proliferation kit for imaging
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Click-iT EdU labeling was conducted using the Click-iT EdU Alexa Fluor 488 Cell Proliferation Kit for Imaging (ThermoFisher) according to manufacturer’s instructions. Following 60-minute incubation with EdU label, cells were washed extensively and the media replaced with EV-free complete cell culture media to allow for TMV shedding. TMVs were isolated as described above, and plated on poly-L-lysine coated coverslips for imaging. Cells and TMVs were fixed and subjected to Click-iT reaction according to manufacturer’s recommended protocol before staining with primary and secondary antibodies as described above.
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