Anti ep300

Antibodies used for western blot (WB), immunofluorescence (IF) and chromatin immunoprecipitation (ChIP) were as follows: anti-H3K36me3 (Solarbio no. K003337P, 1:400 for IF), anti-H3K36me2 (Solarbio no. K003336P, 1:400 for IF), anti-H3 (Solarbio no. K107283P, 1:800 for WB), anti-γ-H2A.X (Solarbio no. K006207P, 1:200 for IF, 1:1000 for WB), anti-mCherry (Solarbio no. K200015M, 1:1000 for WB), anti-H4K16ac (Abcam no. ab109463, 1:200 for IF), anti-NSD2 (Abcam no. ab-75359, 1:1000 for WB, 1:200 for IF), anti-PSMA8 (Proteintech no. 4022-1-AP, 1:1000 for WB), anti-GAPDH (Proteintech no. 60004-1-Ig, 1:1000 for WB), anti-PRM2 (Briar Patch Biosciences, Hup2B, 1:200 for IF, 1:800 for WB), anti-WT1 (Abcam no. ab89901, 1:200 for IF), anti-H4K5ac (ABclonal no. A19525, 1:1000 for WB, 1:200 for IF), anti-H4K8ac (ABclonal no. A7258, 1:1000 for WB, 1:200 for IF), anti-EP300 (Abcam no. ab-10485, 1:1000 for WB), anti-H4 (ABclonal no. A1131, 1:1000 for WB) and anti-α-Tubulin (Proteintech no. 66031-1-Ig, 1:1000 for WB).

For co-IP, cell lysates (containing 2×10⁷ cells) were incubated with antibody-conjugated protein A/G magnetic beads (Thermo Fisher Scientific, Inc.) in Western/IP lysis buffer (Beyotime Institute of Biotechnology) at 4°C overnight. Immunoprecipitates isolated with the magnetic beads were washed five times with Western/IP lysis buffer before being subjected to IB. The antibodies used for co-IP were: Anti-Myc (product no. 2276; 1:100; CST), anti-HA (product no. 2367; CST, 1:100), anti-CREB (product no. 9104; 1:100; CST), anti-EP300 (product code ab54984; 1:100; Abcam) and anti-SETDB2 (product code ab13712; 1:50; Abcam).