T cells were washed and incubated with antibodies at 4 °C for surface staining (see SI Appendix for antibodies) and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody, fixed, and analyzed. To stain for intracellular antigens, cells were fixed with 4% paraformaldehyde and permeabilized with CytoPerm buffer (Biolegend). Cells were analyzed on a BD LSR Fortessa X-20 cytometer. Compensation was performed using CompBeads (BD) and calculated by FACSDiva software. Data were analyzed using FlowJo software.
Cell viability dye zombie uv
Manufactured by BioLegend
Cell viability dye Zombie UV is a fluorescent reagent used to detect dead cells. It binds to nucleic acids in cells with compromised cell membranes, providing a measure of cell death.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cell viability dye zombie uv
1
Flow Cytometry Immunophenotyping of T Cells
2
Multiparameter Flow Cytometry Analysis
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T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody, fixed and analysed. To stain for intracellular antigens, cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer. Compensation was performed using CompBeads (BD) and calculated by FACSDiva software. Data was analysed using FlowJo software.
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