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Ultramicro nucleic acid protein analyzer

Manufactured by Implen
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Sourced in Germany

The Ultramicro nucleic acid protein analyzer is a laboratory instrument designed to measure the concentration and purity of nucleic acids and proteins in small sample volumes. It utilizes spectroscopic techniques to analyze the samples.

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Lab products found in correlation

Cdna reverse transcription kit, by Vazyme (2 mentions) Sybr green rt qpcr supermix kit, by Vazyme (2 mentions) Cfx96 touch, by Bio-Rad (2 mentions)

2 protocols using ultramicro nucleic acid protein analyzer

1

Total RNA Extraction and Quantitative RT-qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
In order to extract total RNA from quail cerebrum tissue, Trizol reagent was used. RNA concentration was determined by using ultramicro nucleic acid protein analyzer (Implen, N60, Germany), then the RNA was reverse transcribed into cDNA by a cDNA reverse transcription kit (Vazyme, Nanjing). As shown in Table 1, speci c primers were synthesized by Sangon Biotech (Shanghai, China). The gene was combined with the corresponding primer according to the instructions of the SYBR Green RT-qPCR Supermix kit (Vazyme, Nanjing, China). The levels of relative mRNA were analyzed by the Bio-Rad CFX96 touch (Hercules, CA, USA). 2 - ΔΔCT method was used to analysis the data (Li et
Li S., Wu P., Han B., Yang Q., Wang X., Li J., Deng N., Han B., Liao Y., Liu Y, & Zhang Z. (2022). Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction. Environmental toxicology, 37(8).
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2

Total RNA Extraction and Quantitative RT-qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
In order to extract total RNA from quail cerebrum tissue, Trizol reagent was used. RNA concentration was determined by using ultramicro nucleic acid protein analyzer (Implen, N60, Germany), then the RNA was reverse transcribed into cDNA by a cDNA reverse transcription kit (Vazyme, Nanjing). As shown in Table 1, speci c primers were synthesized by Sangon Biotech (Shanghai, China). The gene was combined with the corresponding primer according to the instructions of the SYBR Green RT-qPCR Supermix kit (Vazyme, Nanjing, China). The levels of relative mRNA were analyzed by the Bio-Rad CFX96 touch (Hercules, CA, USA). 2 - ΔΔCT method was used to analysis the data (Li et
Wu P., Han B., Yang Q., Li S., Wang X., Li J., Deng N., Han B., Liao Y., & Zhang Z. (2021). Deltamethrin Induces Apoptosis in Cerebrum Neurons of Quail via Promoting Endoplasmic Reticulum Stress and Mitochondrial Dysfunction.
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