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Rabbit anti tyk2

Manufactured by Abcam

Rabbit anti-TYK2 is a primary antibody that recognizes the Tyrosine Kinase 2 (TYK2) protein. TYK2 is a member of the Janus kinase (JAK) family of non-receptor tyrosine kinases and plays a crucial role in cytokine signaling pathways.

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3 protocols using rabbit anti tyk2

1

PBMC Cytokine Stimulation Western Blot

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PBMCs were stimulated with various cytokines for indicated time. The extracted protein were subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)-conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
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2

Western Blot Analysis of STAT Signaling

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PBMCs were stimulated with various cytokines for indicated time. The extracted protein was subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
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3

PBMC Cytokine Stimulation Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stimulated with various cytokines for indicated time. The extracted protein were subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)-conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
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