HA (#3724) and FLAG (#8146 and #14793) antibodies were purchased from Cell Signaling Technology. β-Actin (sc-4777) antibody was purchased from Santa Cruz Biotechnology. Protease inhibitor cocktail (P8340) and crystal violet (C0775) were purchased from Sigma. Nitro Blue tetrazolium chloride (J60230) was purchased from Alfa Aesar. MEM nonessential amino acids and ECL plus western blotting detection reagent were purchased from Thermo Fisher Scientific. Saponin (S0019-25G) was purchased from TCI America. Sep-Pak C18 cartridge was purchased from Waters.
Saponin s0019 25g
Manufactured by Tokyo Chemical Industry
Saponin (S0019-25G) is a natural compound extracted from various plant sources. It has surface-active properties and is commonly used as a reference standard or additive in biochemical and cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Sep pak c18 cartridge, by Waters Corporation (2 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Ecl plus western blotting detection reagent, by Thermo Fisher Scientific (1 mentions)
Nitro blue tetrazolium chloride, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Ha 3724, by Cell Signaling Technology (1 mentions)
2 protocols using saponin s0019 25g
1
Western Blot Antibody Reagent Procurement
2
Cellular Signaling Pathway Reagents and Antibodies
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Chemicals from commercial sources were obtained in the highest purity available. Trichostatin A (TSA, T8552), protease inhibitor cocktail (P8340), phosphatase inhibitor cocktail (P0044), NAD (NAD100-RO), Puromycin (P8833), Crystal Violet (C0775), low-melting point agarose (A0701) were purchased from Sigma-Aldrich (St. Louis, MO ). The anti-Phospho-Erk1/2 (Thr202/204) (9101), Erk1/2 (4696), Phospho-Akt (Thr308) (9275), Phospho-Akt (Ser473) (9271), Akt (4691), Phospho-SAPK/JNK (Thr182/Tyr185) (4668), SAPK/JNK (9252) antibodies were purchased from Cell Signaling Technology (Danvers, MA ). The anti-β-Actin (sc-4777), Na/K-ATPase (sc-21712), GAPDH (sc-47724), the normal rat IgG (sc-2026) and the goat anti-mouse/rabbit/Rat IgG-horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX ). The anti-Acetyl Lysine antibody (ICP0380) was obtained from ImmuneChem (Cananda). Enzyme-linked chemiluminescence (ECL) plus (32132) western blotting detection reagent, Cy3-conjugated goat anti-rabbit IgG (H + L) secondary antibody (A10520) and the high capacity Streptavidin agarose (20357) were purchased from Thermo Fisher Scientific (Waltham, MA ). Saponin (S0019-25G) was from TCI America (Portland, OR ). Sep-Pak C18 cartridge was purchased from Waters (Milford, MA ).
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