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Diff quik cell staining kit

Manufactured by Sysmex
Sourced in Japan

The Diff-Quik cell-staining kit is a quick and reliable method for differentiating and identifying various cell types in clinical and research samples. It utilizes a series of stains to highlight the cellular structures, enabling efficient microscopic examination and analysis.

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2 protocols using diff quik cell staining kit

1

In vitro invasion assay with FABP4

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An in vitro invasion assay was performed in triplicate using Growth Factor Reduced BD BioCoat Matrigel Invasion Chambers (BD Biosciences) according to the manufacturer’s instructions. Briefly, 3 × 104 cells were seeded in the upper chamber with conditioned medium from PrSC treated with or without 100ng ml-1 rFABP4, and the presence or absence of 10  μg ml-1 of IL-8 blocking antibody, IL-6 blocking antibody, control goat IgG, or control mouse IgG (R&D Systems, Minneapolis, MN, USA). In the siRNA experiments, cells were treated with 50 nM FABP4 siRNAs for 24 hours before being seeded in the chambers. Subsequently, 20% FBS DMEM was placed in the lower chamber, followed by incubation for 24 hours. In some experiments, 1 × 104 PrSC were seeded in the lower chamber with optimal medium. Sera from mice were collected, clarified by filtration (SLGV004SL; Millipore, Billerica, MA, USA), and used for ex vivo cell invasion assays. Briefly, 3 × 104 cells were seeded in the upper chamber with medium containing 5% FBS or 5% mouse serum with or without 10  μg ml-1 of IL-8 blocking antibody or 30 μM of BMS309403. DMEM with 20% FBS was placed in the lower chamber. Then, the non-invading cells in the upper chamber were removed and the membranes were stained with a Diff-Quik cell-staining kit (Sysmex, Kobe, Japan) to count the invading cells. The experiments were performed twice each in triplicate.
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2

Measuring Prostate Cancer Cell Invasion

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The in vitro invasion assay was performed in triplicate using growth factor‐reduced BD BioCoat Matrigel Invasion Chambers (BD Biosciences) according to the manufacturer's instructions. Briefly, PCa cells were pretreated with 50 nmol/L siGFRAL for 12 h, then 5 × 104 cells were seeded in the upper chamber and treated with or without 50 ng/mL rMIC‐1. DMEM containing 20% FBS was then placed in the lower chamber and incubated for 24 h. Following incubation, the non‐invading cells in the upper chamber were removed, and the membranes were stained with a Diff–Quik cell‐staining kit (Sysmex, Kobe, Japan) to count the total number of invading cells under a light microscope.
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