Xn 1000v

CBC was measured using a CBC analyzer (Sysmex, #XN-1000 V) to determine the changes in the number of leukocytes in the vehicle group subjected to the drug discontinuation regimen and the levels of CKD-506 and fingolimod in the blood of the vehicle groups subjected to the drug discontinuation and drug change regimens.

Demographics for the 18 participants in the study are shown in S1 Table and in a schematic of the Schedule of Assessments. A total volume of 95.5 mL of blood was collected per donor and distributed into eight tubes (K₂-EDTA, 10 mL lavender/H, #367525 BD Vacutainer) and four EDTA tubes (K₂-EDTA, 3 mL lavender/H, #367838 BD Vacutainer) for cell differentials (CBC) at 5 time points: 3h (baseline), 6h, 24h, 48h & 72h, flow cytometry analysis (at 3, 24, 48 & 72h) and plasma collection (all time points), and in one 3.5 mL serum separation tube (Serum separator tubes, SST™ II advance, BD Vacutainer® ref 368498 (Becton Dickinson Sweden AB)) for serum collection (baseline (3h) only). The 3 mL EDTA tubes were transferred at ambient temperature to the Hematology unit, at Sahlgrenska University Hospital (Gothenburg, Sweden), for CBC analysis on the Abbott CELL-DYN Sapphire hematology analyzer. The eight 10 mL EDTA test tubes per donor were transferred at ambient temperature to the Bioscience department at AstraZeneca (Gothenburg) to be analyzed on the Siemens ADVIA 2120i, Beckman Coulter DxH900, and Sysmex XN-1000V hematology analyzers. Two 10 mL EDTA tubes from each donor were placed at four different temperatures: in the refrigerator (at 4°C), in a box on the bench (room temperature (RT) of 20°C), and in heating cabinets (at 30 and 37°C).