Cell culture plastics

Cell culture plastics, collagenase II, phosphate buffered saline (PBS), fetal calf serum (FCS), DMEM medium and cell culture supplements were purchased from PAA Laboratories GmbH (Pasching, Austria). GeneJET RNA Purification Kit, DNase I (RNase-free) and First Strand cDNA Synthesis Kit were purchased from Fermantas (Ontario, Canada). Oct-4A (C30A3) Rabbit mAB, Sox2 (D6D9) Rabbit mAB, Nanog (D73G4) Rabbit mAB, Lin28A (D84C11) Rabbit mAB and the corresponding secondary Anti-rabbit IgG, HRP linked Antibody were purchased from Cell Signaling (Beverly, USA). Ki67 (M-19) Goat pAB was purchased from Santa Cruz Biotechnology Inc. (Heidelberg, Germany). Chemicals as well as the Anti-TET2, Anti-TET3 and Anti-GAPDH Rabbit pAB were purchased from Sigma (Munich, Germany).

Cell culture medium, DMEM medium, William’s medium E, and cell culture supplements were purchased from Sigma-Aldrich (Steinheim, Germany). Cell culture plastics, phosphate buffered saline (PBS), and fetal calf serum (FCS) were purchased from PAA Laboratories GmbH (Pasching, Austria). DNaseI (RNase—free) and first strand cDNA Synthesis Kit were purchased from Fermantas (Ontario, Canada). 5-Azacytidine (SLBH7350V) was obtained from Sigma-Aldrich (Steinheim, Germany). All other chemical compounds were purchased from Carl Roth (Karlsruhe, Germany). 5hmC (39769) rabbit pAB and 5mC (39649) mouse mAB were purchased from Active Motif (Carlsbad, CA, USA). Proliferating cell nuclear antigen (PCNA) (ab92552) rabbit mAB was obtained from Abcam (Cambridge, UK). Corresponding secondary antibodies goat anti-rabbit Alexa 555 and goat anti-mouse 488 were acquired from Invitrogen (Carlsbad, CA, USA). Anti-TET2, anti-TET3, and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were used from Sigma-Aldrich (Munich, Germany). The HRP-linked anti-rabbit IgG secondary antibody was purchased from Cell Signaling (Beverly, MA, USA).

DMEM medium and cell culture supplements were purchased from Sigma (Steinheim, Germany). Cell culture plastics, phosphate-buffered saline (PBS), and fetal calf serum (FCS) were purchased from PAA Laboratories GmbH (Pasching, Austria). DNase I (RNase-free) and First Strand cDNA Synthesis Kit were purchased from Fermentas (Ontario, Canada). 5-AZA and L-ascorbic acid 2-phosphate were obtained from Sigma-Aldrich (Steinheim, Germany). All other chemical compounds were purchased from Carl Roth (Karlsruhe, Germany). 5-hmC (39769) mouse mAB was purchased from Active Motif (Carlsbad, CA, USA). Proliferating cell nuclear antigen (PCNA) (ab92552) rabbit mAB was obtained from Abcam (Cambridge, UK). Corresponding secondary antibodies goat anti-rabbit Alexa 555 and goat anti-mouse 488 were acquired from Invitrogen (Carlsbad, CA, USA). Anti TET2 (SAB3500711), anti TET3 (SAB2700682), and anti glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G9545) antibodies were used from Sigma (Munich, Germany). GADD45B (SC33172) rabbit mAB was from Santa Cruz, and Snail (3879) rabbit mAB, E-cadherin (14472) mouse mAB, p21 (2947) rabbit mAB, cyclin-B1 (4138) rabbit mAB, and HRP-linked anti-mouse and anti-rabbit IgG secondary antibody were purchased from Cell Signaling (Beverly, MA, USA).