Ms basal salt mixture

Manufactured by Duchefa Biochemie

MS) basal salt mixture is a powdered mixture of inorganic salts and nutrients commonly used as a base medium for plant cell and tissue culture applications. Its core function is to provide the essential mineral elements required for plant growth and development in a controlled and standardized environment.

Automatically generated - may contain errors

Lab products found in correlation

Evans blue, by Merck Group (1 mentions) Myo inositol, by Duchefa Biochemie (1 mentions) Phytoagar, by Duchefa Biochemie (1 mentions)

Close spelling variants of this product

Basal Salt Mixture MS basal salts MS salts

6 protocols using ms basal salt mixture

Micro-Tom Tomato Seed Sterilization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tomato (Solanum lycopersicum cv. Micro-Tom) seeds were separated from the harvested tomato, soaked in 1.2% protease solution (Rapidase) for 1.5 h, and then rinsed with water. Subsequently, the seeds were soaked in 1.6% sodium hypochlorite (NaOCl) for 10 min, rinsed in water, dried thoroughly, and stored until use.
The seeds were sterilized in 70% (v/v) EtOH for 1 min, soaked in 0.8% NaOCl solution for 1–3 min, and then rinsed thoroughly with sterilized, distilled water. After surfacesterilization, the seeds were kept in the dark for an initial 3-day period at 4 °C and thereafter placed onto Murashige and Skoog (MS) basal salt mixture (Duchefa, The Netherlands) supplemented with 3% (w/v) sucrose, 0.4 mg L⁻¹ thiamine, 100 mg L⁻¹ myo-inositol, and 0.4% (w/v) Gelrite, pH 5.8 with 1 N KOH, and cultured under 70% relative humidity and light at approximately 30 µmol m⁻² s⁻¹ (light/dark regime of 16/8 h) at 25 °C.

Lee M.H., Lee J., Jie E.Y., Choi S.H., Jiang L., Ahn W.S., Kim C.Y, & Kim S.W. (2020). Temporal and Spatial Expression Analysis of Shoot-Regeneration Regulatory Genes during the Adventitious Shoot Formation in Hypocotyl and Cotyledon Explants of Tomato (CV. Micro-Tom). International Journal of Molecular Sciences, 21(15), 5309.

+ Open protocol

+ Expand

Thiosulfinate-Induced Cell Death Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

BY-2 cells (kindly provided by Dr. C. Langenbach, Institut f. Biologie III, RWTH Aachen University) were grown in modified MS Medium (4.3 g L⁻¹ MS basal salt mixture (Duchefa Biochemie, Haarlem, Netherlands), 30 g L⁻¹ sucrose, 0.2 g L⁻¹ KH₂PO₄, 0.2 mg L⁻¹ 2,4-Dichlorophenoxyacetic acid, 1 mg L⁻¹ Thiamin Hydrochloride, 100 mg L⁻¹ Myo-Inositol, pH = 5.8 with KOH (all chemicals were purchased from Carl Roth, Karlsruhe, Germany)) shaken in the dark at 90 revolutions min⁻¹ for 7 days. Cells were treated with thiosulfinates for 1 hour and then incubated for 15 min with 0. 5% Evans Blue (Sigma), unbound dye was removed by washing. Dye bound to dead cells was solubilized in 50% methanol with 1% SDS for 30 min at 50 °C and quantified by absorbance at 600 nm in a plate reader. Negative controls were not treated with thiosulfinates and positive controls were heated to 99 °C for 30 minutes^{54 (link)}. Negative and positive controls were set as 0% and 100%, respectively. Data are presented as means with standard deviations of four replicates.

Leontiev R., Hohaus N., Jacob C., Gruhlke M.C, & Slusarenko A.J. (2018). A Comparison of the Antibacterial and Antifungal Activities of Thiosulfinate Analogues of Allicin. Scientific Reports, 8, 6763.

+ Open protocol

+ Expand

Surface Sterilization and Seed Germination

Check if the same lab product or an alternative is used in the 5 most similar protocols

A. thaliana Col-0 WT seeds were surface sterilized with chlorine gas in an airtight glass container for 2 h. Seeds were sown on ½× Murashige and Skoog (MS) basal salt mixture (Duchefa) medium (½× MS, 1% sucrose, 0.7% plant agar, pH 5.6 adjusted with KOH) and incubated in the dark at 4 °C for 1 day before being used in the experiments described below.

Havshøi N.W., Nielsen J, & Fuglsang A.T. (2024). The mechanism behind tenuazonic acid-mediated inhibition of plant plasma membrane H+-ATPase and plant growth. The Journal of Biological Chemistry, 300(4), 107167.

+ Open protocol

+ Expand

Optimizing Cotyledon and Hypocotyl Shoot Regeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine optimal shoot regeneration and gene expression in cotyledon and hypocotyl explants, 4-, 7-, and 10-day-old seedlings were excised after germination (hereafter referred to as day-after-germination: DAG4, DAG7, and DAG10, respectively), divided into three segments, and cultured onto shoot induction medium (SIM) (MS basal salt mixture (Duchefa, The Netherlands)) containing several combinational treatments of 1–2 mg L⁻¹ Zeatin, 0.1–0.2 mg L⁻¹ IAA, 2% or 3% sucrose, 0.4 mg L⁻¹ thiamine, 100 mg L⁻¹ myo-inositol, and 0.4% (w/v) Gelrite, pH 5.8 with 1 N KOH for the indicated time at equal intervals (approximately 2 cm distance). For each treatment, three replicates of nine cotyledon explants were placed in a Petri dish (90 × 90 mm) except for those used for a density experiment (Figure S3). All cotyledons were tested in all abaxial orientations except for those used in an orientation experiment (Figure S2). All cultures were incubated under 70% relative humidity and light at approximately 30 µmol m⁻² s⁻¹ (light/dark regime of 16/8 h) at 25 °C. The efficiency of adventitious shoots formation was calculated with the ratios from the total number of explants and the number of explants showing shoot formation.

+ Open protocol

+ Expand

Sterilization of Tobacco and Tomato Seeds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tobacco (Nicotiana benthamiana) seeds were sterilized with 70% (v/v) ethanol for 0.5 min and soaked in 0.8% sodium hypochlorite (NaOCl) solution for 0.5 min and then washed with sterile distilled water three times. Tomato (Solanum lycopersicum cv. Micro-Tom) seeds were sterilized with 70% (v/v) ethanol for 3 min and 0.8% sodium hypochlorite (NaOCl) solution for 3 min and then washed with sterile distilled water three times. The sterilized tomato seeds were kept in the dark for an initial 3-day period at 4 °C in water. The sterilized seeds were placed on Murashige and Skoog (MS) basal salt mixture (Duchefa, The Netherlands) supplemented with 3% (w/v) sucrose, 0.4 mg L⁻¹ thiamine-HCl, 100 mg L⁻¹ myo-inositol, and 0.4% (w/v) Gelrite, pH of the medium was adjusted to 5.8 with 1 N KOH, and the seeds were cultured in a growth chamber at 25 °C under a 16 h photoperiod (∼30 µmol m⁻² s⁻¹ cool white fluorescent light) and 70% relative humidity.

Lee M.H., Lee J., Choi S.H., Jie E.Y., Jeong J.C., Kim C.Y, & Kim S.W. (2020). The Effect of Sodium Butyrate on Adventitious Shoot Formation Varies among the Plant Species and the Explant Types. International Journal of Molecular Sciences, 21(22), 8451.

+ Open protocol

+ Expand

Dual Ca2+ Sensor Expression in Arabidopsis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate transgenic Arabidopsis thaliana (Col-0) lines that express two Ca²⁺ sensors simultaneously, the cytosolic yellow cameleon reporter line NES-YC3.6 (Krebs et al., 2012 (link)) was transformed with the binary vector barII-UT-R-GECO1, according to standard procedures (Hellens et al., 2000 (link)). Transgenic lines were selected for BASTA resistance on plates containing 10 μg/ml BASTA. For in vitro culture, seeds were surface sterilized using EtOH followed by stratification for 48 h at 4°C. Seedlings were grown at 22°C with cycles of 16 h light and 8 h darkness on plates containing half strength Murashige and Skoog (MS) basal salt mixture (Duchefa; www.duchefa-biochemie.com) supplemented with 0.5% sucrose. Medium pH was set to 5.8 using KOH and medium was solidified using 0.5% phytoagar (Duchefa). Pollen germination was performed as described previously (Hicks et al., 2004 (link)).

Keinath N.F., Waadt R., Brugman R., Schroeder J.I., Grossmann G., Schumacher K, & Krebs M. (2015). Live Cell Imaging with R-GECO1 Sheds Light on flg22- and Chitin-Induced Transient [Ca2+]cyt Patterns in Arabidopsis. Molecular plant, 8(8), 1188-1200.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!