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Bexarotene

Manufactured by LC Laboratories
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Bexarotene is a research chemical product offered by LC Laboratories. It is a retinoid compound commonly used in scientific research applications. The core function of Bexarotene is to serve as a research tool for investigators studying cellular processes and signaling pathways.

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Lab products found in correlation

Uvi3003, by Bio-Techne (3 mentions) Neobee oil, by Thermo Fisher Scientific (3 mentions) T0901317, by Cayman Chemical (2 mentions) 5002 rodent chow, by Land O'Lakes (2 mentions) Ain93m, by Bio-Serv (2 mentions) Kato 3 cells, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Puromycin, by Merck Group (1 mentions) Phenylhydrazine, by Merck Group (1 mentions) Tesaglitazar, by Bio-Techne (1 mentions) Pioglitazone, by Bio-Techne (1 mentions) G csf, by Amgen (1 mentions) Gw7647, by Bio-Techne (1 mentions) 5 fluorouracil, by Merck Group (1 mentions) Gw6471, by Bio-Techne (1 mentions) Pe cy7, by Thermo Fisher Scientific (1 mentions) Humidified chamber, by Embrient Inc (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions) Methocult m3534, by STEMCELL (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Cystamine dihydrochloride, by Thermo Fisher Scientific (1 mentions)

16 protocols using bexarotene

1

Differentiation of OLP6 and KATO-III Cells

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OLP6 cells (RIKEN BioResource Center Cell Bank, Tsukuba, Japan) were incubated in a humidified atmosphere with 5% CO2 at 33 °C in the proliferative stage. Then, they were incubated with 5% CO2 at 39 °C for 4 days to induce differentiation. KATO-III cells (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan) were incubated in a humidified atmosphere with 5% CO2 at 37 °C. Bezafibrate (Ppar/PPAR (peroxisome proliferator-activated receptor) pan-agonist: Sigma-Aldrich, St. Louis, MO, USA) and bexarotene (Rxr/RXR (retinoid X receptor) pan-agonist: LC Laboratories, Woburn, MA, USA) were solubilized in DMSO (dimethyl sulfoxide). Stock solutions at 10 mm were stored at −20 °C until use. Stock solutions were added to cell culture medium to yield final working concentrations of 0.1, 0.3 and 1 μm (bexarotene; OLP6), 5, 15 and 50 μm (bezafibrate; OLP6), 3, 10 and 30 μm (bexarotene; KATO-III) and 15 and 150 μm (bezafibrate; KATO-III). See Supplementary Methods for detailed information.
Maekawa M., Watanabe A., Iwayama Y., Kimura T., Hamazaki K., Balan S., Ohba H., Hisano Y., Nozaki Y., Ohnishi T., Toyoshima M., Shimamoto C., Iwamoto K., Bundo M., Osumi N., Takahashi E., Takashima A, & Yoshikawa T. (2017). Polyunsaturated fatty acid deficiency during neurodevelopment in mice models the prodromal state of schizophrenia through epigenetic changes in nuclear receptor genes. Translational Psychiatry, 7(9), e1229-.
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2

C2C12 Myoblast Differentiation Using Bexarotene

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C2C12 murine myoblasts (ATCC) were maintained in growth medium (GM), Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), in the incubator at 37 °C with 5% CO2. Myogenic differentiation was induced with 80% confluent myoblasts cultures using differentiation medium (DM), DMEM supplemented with 2% horse serum. Bexarotene was purchased from the LC Laboratories.
Li Q., Mach Y.Z., Hamed M., Khilji S, & Chen J. (2023). Regulation of HDAC11 gene expression in early myogenic differentiation. PeerJ, 11, e15961.
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3

Murine Myoblast Isolation and Differentiation

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Mouse primary myoblasts were isolated and differentiated as previously described (AlSudais et al., 2016 (link)). C2C12 myoblasts acquired from the American Type Culture Collection (ATCC) were maintained in growth medium (GM), Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (P/S), at 37°C with 5% CO2. For differentiation, GM of 80% confluent C2C12 culture was replaced with differentiation medium (DM), DMEM supplemented with 2% horse serum, for the indicated duration and treatment. Bexarotene (Gniadecki et al., 2007 (link)) was purchased from the LC Laboratories, UVI3003 (Nahoum et al., 2007 (link)) from the Tocris and puromycin from the Sigma.
Hamed M., Chen J, & Li Q. (2022). Regulation of Dystroglycan Gene Expression in Early Myoblast Differentiation. Frontiers in Cell and Developmental Biology, 10, 818701.
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4

C2C12 Myoblast Differentiation Protocol

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C2C12 mouse myoblasts (ATCC) were maintained in growth medium (GM), consisting of Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin, at 37 °C with 5% CO2. To induce differentiation, cells at 70–80% confluence were switched to differentiation medium (DM), DMEM supplemented with 2% horse serum, and cultured for 24 h. Bexarotene (LC Laboratories) was added to DM at a final concentration of 50 nm for treatment.
Khilji S., Hamed M., Chen J, & Li Q. (2020). Dissecting myogenin-mediated retinoid X receptor signaling in myogenic differentiation. Communications Biology, 3, 315.
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5

Bexarotene and ATRA Receptor Activation

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Bexarotene was from LC Laboratories. ATRA was from Sigma Aldrich. The ApoA1-Luciferase and pBABE-RXRA plasmids were a gift from Vivek Arora, Washington University. MSCV–Gal4 DBD–RXRA LBD–IRES–mCherry (Gal4-RXRA) has been previously described [82 (link)].
Jurutka P.W., di Martino O., Reshi S., Mallick S., Sabir Z.L., Staniszewski L.J., Warda A., Maiorella E.L., Minasian A., Davidson J., Ibrahim S.J., Raban S., Haddad D., Khamisi M., Suban S.L., Dawson B.J., Candia R., Ziller J.W., Lee M.Y., Liu C., Liu W., Marshall P.A., Welch J.S, & Wagner C.E. (2021). Modeling, Synthesis, and Biological Evaluation of Potential Retinoid-X-Receptor (RXR) Selective Agonists: Analogs of 4-[1-(3,5,5,8,8-Pentamethyl-5,6,7,8-tetrahyro-2-naphthyl)ethynyl]benzoic Acid (Bexarotene) and 6-(Ethyl(4-isobutoxy-3-isopropylphenyl)amino)nicotinic Acid (NEt-4IB). International Journal of Molecular Sciences, 22(22), 12371.
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6

C2C12 Myoblast Differentiation with Bexarotene

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Cells of the murine myoblasts cell line C2C12 (ATCC) were maintained in growth medium (GM), Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), at 37°C with 5% CO2. To induce differentiation, the medium of 80% confluent cell cultures was changed to differentiation medium (DM), DMEM supplemented with 2% HS (29 (link)) and 50 nM of Bexarotene was used for treatment conditions. Bexarotene was purchased from the LC Laboratories and UVI 3003 was from Tocris. The RXRα shRNA knockdown cells have been described previously (28 (link)).
Hamed M., Khilji S., Dixon K., Blais A., Ioshikhes I., Chen J, & Li Q. (2017). Insights into interplay between rexinoid signaling and myogenic regulatory factor-associated chromatin state in myogenic differentiation. Nucleic Acids Research, 45(19), 11236-11248.
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7

Bexarotene Modulation of Lipid Metabolism

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Bexarotene was from LC laboratories. Phenylhydrazine, 5- Fluorouracil, corn oil, hexane, isopropanol, were from Sigma. GCSF was from Amgen. GW6471, GW7647, pioglitazone, and tesaglitazar were from Tocris. Anti-mouse CD11b (M1/70)-BV421, anti-mouse c-Kit (2B8)-BV421, anti-mouse B220 (RA3-6B2)-PE-Cy7 were from BD Bioscience. Anti-mouse CD8 (53-6.7)-eFluor450, anti-mouse CD71 (R17217) –eFluor450, anti-mouse Sca-1 (D7)-APC, anti-mouse Gr-1 (RB6-8C5) –APC, anti-mouse CD4 (GK1.5) –APC, anti-mouse Ter119 (TER119) – APC, anti-mouse c-Kit (2B8) –PE-Cy7, anti-mouse CD19 (eBio1D3) –PE-Cy7, anti-mouse Ter119 (TER119)-PE-Cy7, anti-mouse CD127 (ATR34) PE-Cy7, anti-mouse CD8 (53-6.7) –PE-Cy7, anti-mouse CD4 (RM4-5) –PE-Cy7, anti-mouse CD3e (145-2C11) –PE-Cy7 were from eBioscience. Sera from Mouse, Hamster, Rabbit, Rat, Guinea pig, and Goat were obtained from Equitech-Bio, Inc., and sera was obtained while animals were maintained on a standard diet. C24:4 ((9Z, 12Z, 15Z, 18Z, 21Z)-tetracosa-9,12,15,18,21-tetraenoic acid) and C24:5 ((9Z, 12Z, 15Z, 18Z, 21Z)-tetracosa-9,12,15,18,21-pentaenoic acid) were synthesized from Avanti Polar Lipids. Inc. The DR1-Luc reporter and pBABE-RXRA plasmids were gifts from Vivek Arora, Washington University.
Niu H., Fujiwara H., di Martino O., Hadwiger G., Frederick T.E., Menéndez-Gutiérrez M.P., Ricote M., Bowman G.R, & Welch J.S. (2017). Endogenous Retinoid X Receptor ligands in mouse hematopoietic cells. Science signaling, 10(503), eaan1011.
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8

Cryopreserved Leukemic Spleen Cell Assay

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Cryopreserved leukemic spleen cells were thawed, plated at 2 × 106/ml in RPMI with 15% FCS, 100 ng/ml SCF, 6 ng/ml IL-3, 10 ng/ml IL-6 (Peprotech, Rocky Hill, NJ) ± 1 μM ATRA (Sigma, St. Louis, MO) or 1 μM bexarotene (LC Laboratories, Woburn, MA) and maintained at 3% oxygen and 5% CO2 in a humidified chamber (Billups-Rothenberg, Del Mar, CA) for 48 hours. Cells were plated at 8.3 × 103/ml (MethoCult M3534 Stem Cell Technologies, Vancouver, Canada) and maintained in 3% oxygen and 5% CO2. After seven days, colonies were counted.
Welch J.S., Niu H., Uy G.L., Westervelt P., Abboud C.N., Vij R., Stockerl-Goldstein K.E., Jacoby M., Pusic I., Schroeder M.A., Dipersio J.F, & Cashen A.F. (2014). A phase I dose escalation study of oral bexarotene in combination with intravenous decitabine in patients with AML. American journal of hematology, 89(8), E103-E108.
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9

Synthesis and Characterization of MSU42011

Cited 1 time
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MSU42011 was synthesized (>95% purity) as described [22 (link)]. Bexarotene (>99% purity) was acquired from LC Laboratories.
Leal A.S., Moerland J.A., Zhang D., Carapellucci S., Lockwood B., Krieger-Burke T., Aleiwi B., Ellsworth E, & Liby K.T. (2021). The RXR Agonist MSU42011 Is Effective for the Treatment of Preclinical HER2+ Breast Cancer and Kras-Driven Lung Cancer. Cancers, 13(19), 5004.
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10

Molecular Regulation of Phagocytosis

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Transcriptional activation of RAR‐reporter constructs by tRA was found to be maximal between 0.1 and 10 μM (Allenby et al. 1993), and experiments with glial cultures showed strong effects with 0.01–1 μM (van Neerven, Regen, et al., 2010). To test the molecular regulation of phagocytosis we therefore tested 10 nM, 0.1 μM, and 0.5 μM of the pan‐RAR agonist all‐tRA (R2625; Sigma); 0.1 and 0.5 μM pan‐RXR agonist bexarotene (153559‐49‐0; LC Laboratories); 1 μM pan‐RXR antagonist UVI3003 (3303; Tocris); 1 μM RARβ agonist‐RARα/γ antagonist BMS189453 (SML1149; identical to BMS453; Sigma); 1 and 10 μM pan LXR agonist T0901317 (Cay71810‐10; Cayman); 9 and 18 μM PPARα agonist fenofibrate (Cay10005368‐1; Cayman); 50 nM and 0.1 μM PPARγ antagonist rosiglitazone (LKT‐R5773.100; LKT Laboratories); 0.2 and 0.5 μM PPARβ/δ agonist GW501516 (Cay10004272‐1; Cayman); 1 and 2 μM FXR agonist GW4064 (Cay10006611‐5; Cayman); 0.5 and 1 μM TGM2 inhibitor cystamine dihydrochloride (B22873.14; Alfa Aesar); 15, 25, 50, and 100 μM TGM2 inhibitor ERW1041E (5095220001; Merck); and 1, 5, and 25 μM blocker of scavenger receptor CD36 sulfo‐N‐succinimidyl oleate (SSO; SML2148; Merck).
Wu S., Romero‐Ramírez L, & Mey J. (2020). Retinoic acid increases phagocytosis of myelin by macrophages. Journal of Cellular Physiology, 236(5), 3929-3945.
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