CRC cell lines (HCT116, SW-620, LOVO, HCT-15 and SW480) and normal epithelial cell lines, CCD-18Co, were procured from the American Type Culture Collection (ATCC). HCT116 cells (cat. no. CCL-247) were maintained in McCoy's 5a medium (cat. no. 30-2007; ATCC); SW-620 (cat. no. CCL-227) and SW480 cells (cat. no. CCL-228) were grown in Leibovitz's L-15 medium (cat. no. 30-2008; ATCC); LOVO cells (cat. no. CCL-229) were maintained in F-12K medium (cat. no. 30-2004; ATCC); HCT-15 cells (cat. no. CCL-225) were cultured in RPMI-1640 medium (cat. no. 30-2001; ATCC); and CCD-18Co cells (cat. no. CRL-1459) were maintained in Eagle's Minimum (cat. no. 30-2003; ATCC). All cells were cultured in media supplemented with 10% FBS (cat. no. 16000044; Gibco; Thermo Fisher Scientific, Inc.) in a humidified containing 5% CO2 at 37°C.
Sw620
Manufactured by American Type Culture Collection
Sourced in United States, China, Germany, United Kingdom, France, Australia, Israel, Austria
The SW620 is a type of cell line maintained by the American Type Culture Collection (ATCC). It is a human colorectal adenocarcinoma cell line derived from a metastatic site. The SW620 cell line is commonly used in cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by American Type Culture Collection (812 mentions)
Sw480, by American Type Culture Collection (811 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (408 mentions)
Dld 1, by American Type Culture Collection (273 mentions)
Caco 2, by American Type Culture Collection (200 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (167 mentions)
Ht 29, by American Type Culture Collection (146 mentions)
Ncm460, by American Type Culture Collection (125 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (119 mentions)
Sw620, by Thermo Fisher Scientific (118 mentions)
Hct15, by American Type Culture Collection (116 mentions)
Ls174t, by American Type Culture Collection (113 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (95 mentions)
Sw480, by Thermo Fisher Scientific (92 mentions)
Hct116, by Thermo Fisher Scientific (90 mentions)
Penicillin, by Thermo Fisher Scientific (82 mentions)
Streptomycin, by Thermo Fisher Scientific (80 mentions)
Colo205, by American Type Culture Collection (77 mentions)
Rpmi 1640, by Thermo Fisher Scientific (73 mentions)
Hek293t, by American Type Culture Collection (58 mentions)
1 211 protocols using sw620
1
Culturing Colorectal Cancer Cell Lines
2
Culturing of Human Colon Cell Lines
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Normal human colon epithelial cell line (FHC) and five human CRC cell lines (HCT-116, HT29, LoVo, SW480, and SW620) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). FHC cells were cultured in DMEM/F12 medium (ATCC) containing 25 mM HEPES, 10 ng/ml cholera toxin, 5 μg/ml insulin and transferrin, and 100 ng/ml hydrocortisone. LoVo cells, HCT-116 and HT29 cells, and SW480 and SW620 cells were grown in F-12K medium, McCoy’s 5a medium modified, and Leibovitz’s L-15 medium (all ATCC), respectively. All of the media were supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA). Cells were maintained at 37°C in a humidified incubator under 5% CO2 condition10 (link).
3
Culturing Colorectal Cancer Cell Lines
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All CRC cell lines (SW480, SW620, HT-29, COLO 205, and WiDr) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in Dulbecco's Modified Eagle Medium (DMEM; Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone) and 10 U/mL of penicillin-streptomycin (Hyclone). All cell lines were routinely tested for mycoplasma contamination and cell lines used in subsequent experiments (SW480 and SW620) were authenticated by short tandem repeat profiling at the Research Institute of National Cancer Center (Republic of Korea).
HUVEC cells were purchased from ATCC, and cultured in Endothelial Cell Growth medium (EGM-2; Lonza, Walkersville, MD, USA) supplemented with 10% FBS.
HUVEC cells were purchased from ATCC, and cultured in Endothelial Cell Growth medium (EGM-2; Lonza, Walkersville, MD, USA) supplemented with 10% FBS.
4
Colon Cancer Cell Line Characterization
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Human colon cancer cells HT29, HCT116, and SW620 were obtained from the American Type Culture Collection (ATCC, Manassas, VA). HT29 and HCT116 cells were propagated in complete McCoy’s 5 A media (Sigma, St. Louis, MO) containing 10% fetal bovine serum (FBS) (Peak Serum, Wellington, CO). SW620 cells were grown in Leibovitz L-15 media (ATCC) containing 10% FBS. Mouse colon cancer CMT93 cells (ATCC) were propagated in DMEM (Sigma) containing 10% FBS. Human non-transformed colonic NCM460 cells were obtained from INCELL Corporation (INCELL, San Antonio, TX) and were grown in an M3Base medium (INCELL) containing 10% FBS. All colon cancer cell lines have been authenticated by ATCC through a short tandem repeat (STR) DNA profile and were tested for mycoplasma contamination. Cells were serum starved overnight prior to experimental procedures and were serum starved for three days to synchronize cells in the cell cycle prior to treatments.
5
Human Colon Cancer Cell Line Culture and CD137L Signaling Induction
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The four human colon cancer cell lines HT-29, HCT-116, SW480, and SW620 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). HT-29 and HCT-116 cells were cultured in McCoy Medium (ATCC) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (pen/strep). SW480 and SW620 cells were maintained in RPMI 1640 medium (ATCC) containing 10% (v/v) FBS and 1% (v/v) pen/strep. All cell lines were incubated at 37°C in 5% CO2. To induce CD137L signaling cell culture plates were coated with 10 µg/ml recombinant human CD137-Fc (R&D Systems, Minneapolis, MN, USA) at 4°C overnight. To preserve cell surface epitopes cells were then carefully detached using accutase solution (Sigma-Aldrich, St. Louis, MO, USA) and seeded in the pre-coated wells in a defined number.
6
Intestinal Epithelial Cell Lines: Cultivation and Stimulation
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The intestinal epithelial cell lines including HT29, SW620, SW480, LoVo, HCT116, Caco‐2 and IEC‐6 were purchased from American Type Culture Collection, and cultured routinely in RPMI 1640 (HT29, SW620, SW480, LoVo and HCT116) or DMEM (Caco‐2 and IEC‐6) medium respectively, supplemented with 10% or 20% FBS at 37°C in 5% CO2. Cultured or transfected cells were incubated with 1 μM PMA for 15 min. or 1 μg/ml LPS from E. coli for 24 hrs, then processed as follows.
7
Establishing Diverse Cell Line Panel
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The following tumour cell lines were purchased from ECACC directly from Sigma-Aldrich:breast: MDA-MB-231[16 (link)](92020424), MCF7[17 (link)] (86012803); colon: HT29[18 (link)] (91072201), SW480[18 (link)] (87092801), SW620[18 (link)] (87051203); lung: A549[19 (link)] (86012804) and prostate: PC-3[20 (link)] (90112714); as well as endothelial cells: HUVEC (S200-05N) and leucocytes: Jurkat cells[21 (link)] (88042803).The brest cancer tumour cell HCC1937 lines[22 (link)](ATCC-CCL-247)and HCC1954[23 (link)] (ATCC CRL-2338) were purchased from ATCC; the endometrial cell lines HEC1A and HEC1A stably expressing the ETV5 transcription factor (HEC1A-ETV5) were previously described [24 (link)–26 (link)]. Medium for cell culture were obtained from Gibco (DMEM: SW480, SW620, A549, MCF7, MDA-MB-231, PC-3; McCoyy᾿s: HT29, HEC1A, HEC1A-ETV5; RPMI 1640: HCC1937, HCC1954,Jurkat), and from Lonza (Basel, Switzerland) (EGM-2: Huvec). The medium was supplemented with 10% FBS and 1% streptavidin-penicillin (Life Technologies, Carlsbad Ca, USA). Cells were maintained at 37°C in a 5% CO2 incubator. For selected experiments, we transduced MDA-MB-231 and SW480 cells with lentiviral transduction particles (turboGFP, Sigma-Aldrich) to establish cells that expressed green fluorescent protein (GFP); cells were further selected with puromycin (5 μg/mL) and checked by flow cytometry (96%).
8
Characterization of Colorectal Cancer Cell Lines
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The following source of the cell lines were COLO 201, HT29, HCT 116, SW837, SW620, and SW1463 (ATCC); LIM2551, LIM1215, and LIM2099 (Ludwig Institute for Cancer Research, Melbourne, Australia), GEO (29 (link)), DIFI (30 (link)), and V9P (31 (link)). All cell lines were maintained in DMEM/F12 supplemented with 5% FBS (v/v; all from Thermo Fisher Scientific) at 37°C with 5% CO2. Cell lines were authenticated by short tandem repeat profiling using the GenePrint 10 system (Promega), and all were found to be exact matches to published profiles. All cell lines were frozen down as large batches of master stocks within five passages of their purchase from these commercial vendors and confirmed to be Mycoplasma negative at the point of freezing. Experiments were then performed using these master stocks for up to 20 passages. Mycoplasma testing was performed every 3 to 6 months as part of routine monitoring in our laboratory.
9
Cultivation of Colon Carcinoma Cell Lines
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Human colon carcinoma cell lines, namely HT-29, HCT-116, SW480, and SW620, were
purchased from ATCC (Manassas, VA, USA) and cultured in low-glucose Dulbecco’s
modified Eagle medium [5 mMd -(+)-glucose, Gibco-BRL, Gaithersburg, MD,
USA] supplemented with 10% fetal calf serum (Gibco-BRL) and 100 U/ml of penicillin.23 (link) These four human colon carcinoma cell lines are cultured in the same
culture medium to laminate the various nutrients and supplements differences of
the differential medium. The cell lines HT-29, HCT-116, and SW480 were
established from a primary colon adenocarcinoma, and the SW620 cell line was
established from lymph node metastasis in the SW480 patient. The cells were
cultured at 37°C in an atmosphere of 5% CO2.
purchased from ATCC (Manassas, VA, USA) and cultured in low-glucose Dulbecco’s
modified Eagle medium [5 mM
USA] supplemented with 10% fetal calf serum (Gibco-BRL) and 100 U/ml of penicillin.23 (link) These four human colon carcinoma cell lines are cultured in the same
culture medium to laminate the various nutrients and supplements differences of
the differential medium. The cell lines HT-29, HCT-116, and SW480 were
established from a primary colon adenocarcinoma, and the SW620 cell line was
established from lymph node metastasis in the SW480 patient. The cells were
cultured at 37°C in an atmosphere of 5% CO2.
10
Cell Culture and Genetic Manipulation
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SW1116, LoVo, Colo320DM, SW480, SW620 and CT26 cell were from ATCC and cultured in RPMI1640 + 10% FBS. 5-FU was from Sigma. The PERK inhibitor was described previously and purchased from EMD Millipore. The IRE1α inhibitor was purchased from MCE. Lentiviral short hairpin RNA (shRNA) constructs targeting PERK, ATF4, IRE1, GCN2 and PKR were generated as described previously18 (link). Lentiviral integration was selected with 2 µg/ml puromycin for 5 days.
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