Frozen 8-mm sections were washed with PBS, blocked with 5% (w/v) BSA/PBS (1 h at RT), incubated with a primary antibody anti-Xbp1s (1:50; 619502, BioLegend, San Diego, CA), anti- NKCC2 (1:200; NKCC21-A, Alpha Diagnostic International, San Antonio, TX), or anti-NCC (1:200; SPC-402D, StressMarq, Biosciences Inc., Victoria, Canada) at 4°C overnight, and detected by secondary antibodies conjugated with Alexa Fluor 594 or Alexa Fluor 488 (1:500; Molecular Probes, Eugene, OR). Sections stained with Lotus Tetragonolobus Lectin (LTA) were incubated with fluorescein-labeled LTA (1:500; FL-1321, Vector Laboratories, Burlingame, CA) at RT for 30 min before washing. Sections stained with Dolichos Biflorus Agglutinin (DBA) were incubated with biotinylated DBA (1:400; B-1035, Vector Laboratories, Burlingame, CA) at 4°C overnight, and detected by fluorescein Avidin D (1:500; A-2001, Vector Laboratories, Burlingame, CA).
Spc 402d
Manufactured by StressMarq Biosciences
Sourced in Canada
The SPC-402D is a laboratory instrument designed for the study of cellular stress. It features a temperature-controlled incubation chamber and can measure various parameters related to cellular responses under stress conditions. The core function of this product is to provide a controlled environment for conducting stress-related experiments on cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Fl 1321, by Vector Laboratories (1 mentions)
B 1035, by Vector Laboratories (1 mentions)
Avidin d, by Vector Laboratories (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Ab3562p, by Merck Group (1 mentions)
Ab111346, by Abcam (1 mentions)
2 protocols using spc 402d
1
Immunohistochemical Analysis of Key Renal Proteins
2
Antibody-based Analysis of Ion Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies anti‐NKCC2 were from Merck Millipore (AB3562P, Merck Millipore, Burlington, MA). The antibody against the phosphorylated threonine 96 and 101 NKCC2
19 (link) was kindly provided by Prof. Biff Forbush, Yale University. Antibodies anti‐NCC were from StressMarq Biosciences Inc. (SPC‐402D, StressMarq Biosciences Inc., Victoria, BC, Canada). Antibodies against the phosphorylated Thr 53 NCC were from Phosphosolutions (p1311‐53, Phosphosolutions, Aurora, CO). The antibody against human AQP2 was previously described.
20 (link) Antibodies against the phosphorylated 256 AQP2 were from Abcam (ab111346, Abcam, Cambridge, UK). BRL37344 was from Santa Cruz Biotechnology (sc‐200154, Santa Cruz Biotechnology, Dallas, TX).
19 (link) was kindly provided by Prof. Biff Forbush, Yale University. Antibodies anti‐NCC were from StressMarq Biosciences Inc. (SPC‐402D, StressMarq Biosciences Inc., Victoria, BC, Canada). Antibodies against the phosphorylated Thr 53 NCC were from Phosphosolutions (p1311‐53, Phosphosolutions, Aurora, CO). The antibody against human AQP2 was previously described.
20 (link) Antibodies against the phosphorylated 256 AQP2 were from Abcam (ab111346, Abcam, Cambridge, UK). BRL37344 was from Santa Cruz Biotechnology (sc‐200154, Santa Cruz Biotechnology, Dallas, TX).
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