Ec9706

The EC9706 human esophageal squamous cell carcinoma cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Beijing, China). The cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 units/ml penicillin and 100 µg streptomycin/ml (all purchased from Sigma-Aldrich, St. Louis, MO, USA). The cells were incubated at 37°C in a humidified atmosphere containing 95% air and 5% CO₂, and were sub-cultured every three days.

Human ESCC cell lines EC9706, ECa109, and EC1 were obtained from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI/1640 medium with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere consisting of 5% CO₂, as described in our previous study [19 (link)]. Male athymic BALB/c nude mice (Slack King of Experimental Animals Co. Ltd., Wuhan, China) at 4 to 6 weeks of age and 18 to 22 g of weight were used in this study. All the animals were housed in independently ventilated cages (IVC) at a temperature of 25-26°C and a relative humidity of ~50%, lit 12 hours/day. All animal studies were carried out in compliance with the Guide for the Care and Use of Laboratory Animals of Henan Province, China. The data of patient tissue samples were described as in our previous paper [20 (link)].

EC1, EC9706, and HET‐1A cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All cells were maintained in RPMI 1640 medium supplemented with 10% foetal bovine serum (FBS; Gibco BRL, Gaithersburg, MD, USA) and incubated at 37°C and 5% CO₂.