Anti cleaved caspase 3 ab2302

Fetal bovine serum (FBS) and neurobasal cell medium were purchased from Gibco BRL (Grand Island, NY, USA). Brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), papain, poly-L-lysine, forskolin, BSA, collagenase and ovomucoid were purchased from Sigma Chemical Company (St. Louis, MO, USA). B27 supplement was purchased from Invitrogen Life Technologies Company (Carlsbad, CA, USA). Anti-CREB (#9197) and anti-phospho-CREB (p-CREB, #9198) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti–B-cell lymphoma 2 (Bcl-2, #ab182858), anti–B-cell lymphoma extra large (Bcl-xl, #ab32370), anti–caspase 3 (#ab32351), OX-42 (#ab33827), and anti–cleaved caspase 3 (#ab2302) were purchased from Abcam (Cambridge, UK). Anti–tubulin β3 (#801202) and Thy-1.1 (#202508) were purchased from BioLegend (San Diego, CA, USA). DSCR1 (#sc-377507) and Brn3a (#sc-31984) were purchased from Santa Cruz (Dallas, TX, USA). 4',6-Diamidino-2-phenylindole (DAPI) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).