Control Y. pestis cells subcultured without NaCl and Y. pestis cells exposed to 150 g/L NaCl for 7 weeks were examined by electron microscopy using both negative staining and inclusion. Observations were made using a Morgagni 268D microscope (FEI, Philips, France) operating at X35-X280000 magnification. Negative stains were made by contrasting samples with a solution of 1% ammonium molbydate, inclusions were performed after sample fixation in a 2% glutaraldehyde. For each grid, a minimum of 150 Y. pestis cells were observed, and a minimum of five grids were observed for each condition.
Morgagni 268d microscope
Manufactured by Philips
Sourced in France
The Morgagni 268D is a high-performance transmission electron microscope designed for advanced materials analysis. It features a Schottky field emission gun and provides a resolution of up to 0.34 nm, making it suitable for a wide range of research and industrial applications.
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Lab products found in correlation
2 protocols using morgagni 268d microscope
1
Electron Microscopy of Y. pestis Stress
2
Ultrastructural Analysis of Brain Tissue
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Brain tissue samples were fixed in 2% glutaraldehyde in Sorensen buffer pH 7.4 for 2 h, postfixed in 1% osmium tetroxide in aqueous solution (2 h), dehydrated in graded concentrations of acetone and embedded in Epon-Araldite mixture (Electron microscopy Sciences, PA, USA). Ultrathin tissue sections (70 nm) were placed on Cu/Rh grids with Ultracut E (Reichert, Wien, Austria) and were observed using a Morgagni 268D microscope (Philips, The Netherlands).
For imaging the Qtracker ® 800 in solution, a droplet of nanoparticle solution was put on the grid (FCF-150-Cu, Electron Microscopy Science, PA, USA) and, after drying, the grid was imaged without any other processing.
For imaging the Qtracker ® 800 in solution, a droplet of nanoparticle solution was put on the grid (FCF-150-Cu, Electron Microscopy Science, PA, USA) and, after drying, the grid was imaged without any other processing.
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