Candida albicans atcc 14053

Fungal strains were purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) as follows: Aspergillus brasiliensis ATCC 9341 and Candida albicans ATCC 14053 strains. Briefly, the cell suspension of both fungal species was adjusted to 1.0 × 10⁵ CFU/mL in buffered peptone water (BPW) (bioMerieux, Florence, Italy). Peptide stock solutions in DMSO were added to the cell suspension at a final concentration of 25 μM and 50 μM and incubated for 6 h a 37°C. The minimum fungicidal concentration (MFC) was determined by plating 100 μL cultures on DG18 plates (Dichloran 18% Glycerol Agar—ISO 21527-2) for CFU counting. After 7 days at 25°C, the MFC was defined as the lowest peptide concentration that resulted in 99.9% killing compared with the drug-free group. The analyses were performed in triplicate on three different experiments.

Fungal strains used in this study were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) as follows: Aspergillus brasiliensis ATCC 9341 and Candida albicans ATCC 14053 strains. Briefly, for both fungal species, the cell suspension was adjusted to 1.0 × 10⁵ CFU/mL in buffered peptone water (BPW) (bioMerieux, Florence, Italy). Peptide stock solution in DMSO was added to the fungal suspension at a final concentration of 25 µM and 50 µM and incubated for 6 h a 37 °C. The minimum fungicidal concentration (MFC) was determined by plating 100 µL cultures on DG18 plates (Dichloran 18% Glycerol Agar—ISO 21527-2) for CFU counting. After incubation at 25 °C for 7 days, the MFC was defined as the lowest peptide concentration that resulted in 99.9% killing compared with the drug-free group. The analyses were performed in triplicate on three different experiments.